how to prepare acetate buffer solution | Acetate buffer solution is prepared by combining

Summary

This video provides a step-by-step guide on how to prepare an acetate buffer solution. It begins with weighing 34g of sodium acetate and dissolving it in 150-200 mL of distilled water. The next step involves adding 10 mL of 1N acetic acid to the mixture, then transferring the solution to a 250 mL volumetric flask and filling it to the mark with more distilled water. The video explains how to adjust the pH with HCl or sodium hydroxide, and concludes with storing the buffer solution in a storage bottle.

Takeaways

• 😀 The video is a tutorial on how to make a sodium acetate buffer solution.
• 👍 The video starts by welcoming viewers to the YouTube channel and encouraging likes and subscriptions.
• ⚖️ The first step involves using a weight balance to measure 34 grams of sodium acetate.
• 🧪 Sodium acetate is added into 150-200 mL of distilled water and mixed well.
• 🥄 A spatula is used to ensure the mixture becomes homogeneous.
• 🔬 10 mL of acetic acid (1N) is pipetted and poured into a beaker.
• 🥛 The solution is then transferred to a 250 mL volumetric flask using a funnel.
• 💧 Additional distilled water is added to the flask to reach the 250 mL mark.
• 📏 The prepared acetate buffer solution can have its pH adjusted by adding HCl or sodium hydroxide.
• 💼 The buffer solution can be stored in a storage bottle for future use.

Q & A

What is the first step in preparing the acetate buffer solution in the video?

The first step is to turn on the weight balance, place a petri dish on it, and then tare (zero) the balance.

How much sodium acetate should be measured for the buffer solution?

You should measure precisely 34 grams of sodium acetate.

What amount of distilled water is recommended for mixing with sodium acetate?

It is recommended to use between 150 to 200 mL of distilled water to dissolve the sodium acetate.

Why is it important to make the sodium acetate solution homogeneous?

Making the solution homogeneous ensures that the sodium acetate is evenly distributed in the water, which is essential for the proper function of the buffer solution.

What concentration of acetic acid is used in the video for the buffer solution?

A 1 normal (1N) solution of acetic acid is used in the preparation of the buffer.

How much acetic acid is required for the buffer solution preparation?

10 mL of acetic acid is pipetted out and used in the preparation.

What is the purpose of using a volumetric flask in this experiment?

The volumetric flask is used to accurately measure and dilute the buffer solution to a final volume of 250 mL.

How do you ensure that the volumetric flask is filled to the correct level?

You should add distilled water to the flask gradually until the liquid level reaches the 250 mL mark on the flask.

What can be added to the acetate buffer solution to adjust its pH?

Hydrochloric acid (HCl) or sodium hydroxide (NaOH) can be added to the buffer solution to adjust its pH as needed.

What is the final step after preparing the acetate buffer solution?

The final step is to store the prepared acetate buffer solution in a storage bottle for future use.