Video Praktikum Fitokimia Ekstraksi Cair-Cair D3 Farmasi UNS (Anif Nur Artanti)

Summary

This video demonstrates the process of separating phenolic compounds from clove oil in a chemistry experiment. The procedure involves using a separating funnel, NaOH, and petroleum ether to create two distinct layers, with careful attention to safety during the steps. The video emphasizes the importance of gradually adding substances, monitoring pH, and separating layers effectively. It concludes with a polite thank-you and a call to continue practicing laboratory skills. The goal is to provide viewers with a clear, step-by-step guide to performing this chemical separation safely and effectively.

Takeaways

• 😀 The experiment is a practical demonstration for separating phenolic compounds from clove oil using a solvent extraction method.
• 😀 The solvents used in the experiment are NaOH (Sodium Hydroxide) and Petroleum Ether, which help separate the compounds effectively.
• 😀 Essential lab equipment used includes a separating funnel (corong pisah), beaker, stirring rod, porcelain mortar, and water.
• 😀 The clove oil is first placed into the separating funnel, and NaOH is added gradually to begin the separation process.
• 😀 The mixture is then shaken for 10 minutes, and pH testing is performed to monitor the progress of the reaction.
• 😀 Once two distinct layers form, they are separated by carefully draining the bottom layer from the funnel.
• 😀 After separating the layers, HCl is added to the mixture to further separate the phenolic compounds, which causes the formation of two new layers.
• 😀 Petroleum Ether is then added to extract the desired compounds, resulting in the formation of two layers again.
• 😀 It is crucial to ensure the separating funnel is pointed away from people during the shaking process to avoid accidents.
• 😀 The final result yields two layers, with the upper layer being the one containing the phenolic compounds, which is then carefully separated.

Q & A

What is the main goal of the experiment in the script?

The goal of the experiment is to separate phenolic compounds from clove oil using a solvent-based method.

Which solvents are used in the experiment for the separation process?

The experiment uses NaOH (sodium hydroxide) and water as solvents for separating the phenolic compounds from the clove oil.

What laboratory equipment is required for this experiment?

The experiment requires equipment like a separating funnel (corong pisah), beakers (gelas beker), stirring rods (batang pengaduk), porcelain mortar (calon porselin), and pH test strips.

How is the NaOH added to the clove oil in the experiment?

NaOH is added gradually to the clove oil until the mixture reacts and starts to form two layers.

What is the purpose of shaking the mixture for 10 minutes?

Shaking the mixture for 10 minutes helps in the proper mixing and formation of two distinct layers for easier separation.

How is the pH level monitored during the experiment?

The pH level is monitored using pH test strips to ensure that the mixture reaches the appropriate pH for the next steps.

What happens after the two layers form in the mixture?

Once two layers are formed, the upper and lower layers are separated using the separating funnel.

What is added to the lower layer after separating it?

After separating the layers, HCl (hydrochloric acid) is added gradually to the lower layer until it becomes cloudy.

Why is petroleum ether added to the mixture?

Petroleum ether is added to the mixture to help form two separate layers again, which aids in the separation of the phenolic compounds.

What is the significance of ensuring that the tip of the separating funnel is not facing anyone during shaking?

It is important to ensure the tip of the separating funnel is not facing anyone to avoid accidents, as the pressure in the funnel can cause splashes of the chemicals.