Fermentasi Karbohidrat

Summary

The transcript details a fermentation experiment involving a mixture of 1% glucose and 1% sucrose with yeast. It outlines the process of measuring and mixing the ingredients in a mortar until homogenous, then transferring the mixture to fermentation tubes, which are sealed with cotton. After incubating at 36°C for an hour, the final volume is measured to determine the amount of carbon dioxide produced, indicated by the sensation of suction on the skin. This practical demonstration illustrates the fermentation process and the signs of yeast activity, showcasing the experiment's results and significance.

Takeaways

• 😀 The experiment involves fermenting solutions of glucose and sucrose using yeast.
• 👩‍🔬 Yeast is ground into a fine powder to enhance its effectiveness in the fermentation process.
• 📏 Precise measurements are crucial, with 20 mL of each sugar solution being added to the yeast mixture.
• 🔬 After mixing, the resulting solution is placed into fermentation tubes and sealed with cotton to prevent contamination.
• 🌡️ The fermentation tubes are incubated at a temperature of 36°C for one hour to facilitate fermentation.
• 📈 Initial and final volumes of the solution are measured to assess the extent of fermentation.
• 🔍 If the final volume exceeds the tube's scale, a ruler can be used for accurate measurement.
• 💨 Carbon dioxide (CO₂) production is tested by observing skin reactions when sealing the tube openings.
• 🤚 The palm test for CO₂ is conducted by placing the palm over the tube's opening to check for suction effects.
• ✔️ Observing both CO₂ tests confirms the fermentation process and indicates yeast activity.

Q & A

What is the main purpose of the experiment described in the transcript?

The main purpose of the experiment is to demonstrate the fermentation process using yeast and sugar solutions to produce carbon dioxide.

What ingredients are used in the fermentation experiment?

The ingredients used include 1% glucose, 1% sucrose, and yeast (ragi roti).

How is the yeast prepared before mixing with the sugar solutions?

The yeast is ground into a fine powder using a mortar and pestle (lumpang).

What steps are taken to ensure the yeast and sugar solutions are homogeneous?

The glucose or sucrose solution is added gradually to the yeast while stirring until the mixture is uniform.

What is done with the mixture after it is homogenized?

The homogenized mixture is transferred into fermentation tubes, which are then sealed with cotton at the top.

At what temperature and for how long is the fermentation process incubated?

The fermentation is incubated at 36 degrees Celsius for one hour.

How is the final volume of the solution measured after fermentation?

The final volume is measured against the scale on the fermentation tube, and if it exceeds the scale, a ruler can be used for precise measurement.

What indicates the presence of carbon dioxide during the fermentation process?

The presence of carbon dioxide can be tested by feeling the skin on the thumb, which will feel sucked in if carbon dioxide is produced, or by placing the palm over the tube mouth to feel for suction.

What should be done if the volume of the solution exceeds the marked scale on the fermentation tube?

If the volume exceeds the marked scale, a ruler can be used to measure the overflow accurately.

What observation was made regarding the fermentation of sucrose compared to glucose?

The transcript indicates that similar methods are used to measure the final volume for sucrose fermentation, but does not provide specific comparative observations between the two.