Fermentation

Amoeba Sisters
30 Apr 201808:34

Summary

TLDRThe video script explores the importance of oxygen in cellular respiration and how organisms adapt to its absence. It explains the process of ATP production, the misconception about plants needing oxygen, and the shift to fermentation in anaerobic conditions. Examples include alcoholic fermentation by yeast and lactic acid fermentation in muscle cells, emphasizing the limited ATP yield compared to aerobic respiration.

Takeaways

  • 🏊 Swimming was the speaker's favorite childhood activity, and they had a childhood misconception about fish not needing oxygen.
  • 🐟 Most fish use gills to extract oxygen from water, debunking the speaker's early belief that fish don't need oxygen.
  • 🌿 Oxygen is essential for a variety of organisms, including humans, fish, and plants, which counteracts the common misconception about plants not needing oxygen.
  • 🔬 Cells require oxygen for cellular respiration, a process that uses oxygen to break down glucose and produce ATP, the energy currency of cells.
  • 🔋 ATP, or adenosine triphosphate, is a molecule with high energy potential, crucial for powering many cellular functions.
  • ♻️ During cellular respiration, ATP can be converted to ADP and back again, with the help of enzymes, to sustain cellular energy needs.
  • 🌀 When oxygen is absent, certain cells and organisms, like some bacteria, archaea, yeast, and muscle cells, can resort to anaerobic respiration or fermentation to produce ATP.
  • 🍺 Fermentation is an anaerobic process that allows glycolysis to continue by regenerating NAD+, with different products like ethanol in alcoholic fermentation and lactate in lactic acid fermentation.
  • 🍞 Yeast performs alcoholic fermentation to make bread rise, utilizing the carbon dioxide produced and generating a small amount of alcohol that evaporates during baking.
  • 🏋️‍♀️ Lactic acid fermentation occurs in muscle cells under oxygen debt, such as during intense exercise, and may not be the direct cause of post-exercise muscle soreness as previously thought.

Q & A

  • What was the speaker's childhood fascination with swimming?

    -The speaker loved swimming and was on the swim team starting at the age of four. They dreamed of being like a fish and even had a misconception that fish didn't need oxygen.

  • How do most fish obtain the oxygen they need?

    -Most fish have gills that allow them to extract the oxygen they need from the water.

  • What is the role of oxygen in cellular respiration?

    -Oxygen is a reactant in cellular respiration, needed to break down glucose to form ATP (adenosine triphosphate), which powers many cellular processes.

  • Why do plants, which produce oxygen through photosynthesis, still need oxygen?

    -Although plants produce oxygen during photosynthesis, they also need oxygen for cellular respiration, as they perform respiration and thus require oxygen for their own cells.

  • What happens to ATP after it loses a phosphate?

    -When ATP loses a phosphate, it becomes ADP (adenosine diphosphate), which has two phosphates.

  • What is the difference between aerobic and anaerobic cellular respiration?

    -Aerobic cellular respiration requires oxygen, while anaerobic respiration does not. Anaerobic respiration occurs in the absence of oxygen, and organisms like some bacteria and archaea can perform it using alternative electron acceptors.

  • How do muscle cells handle a lack of oxygen during intense exercise?

    -Muscle cells can shift to lactic acid fermentation when oxygen is scarce, such as during intense exercise, allowing them to continue producing ATP through glycolysis.

  • What is fermentation and why is it important for organisms in low oxygen conditions?

    -Fermentation is a process that allows glycolysis to continue and produce ATP in the absence of oxygen. It is important for organisms as it provides a way to generate energy when oxygen is limited.

  • How does alcoholic fermentation differ from lactic acid fermentation?

    -In alcoholic fermentation, pyruvate is converted into acetaldehyde, which then produces carbon dioxide and ethanol. In lactic acid fermentation, pyruvate is converted directly into lactate, which can contribute to muscle soreness.

  • What is the role of NAD+ and NADH in glycolysis and fermentation?

    -NAD+ is an oxidizing agent that is reduced to NADH when it gains electrons during glycolysis. In fermentation, NADH gives its electrons to an electron acceptor to be oxidized back into NAD+, which is necessary for glycolysis to continue.

  • How does the process of fermentation relate to the production of bread and yogurt?

    -Fermentation is used by yeast in bread making to produce carbon dioxide, which helps the bread rise, and by bacteria in yogurt production, where lactic acid contributes to its sour taste.

Outlines

00:00

🐟 The Wonder of Water and the Need for Oxygen

This paragraph introduces the speaker's childhood love for swimming and the dream of being like a fish. It clarifies a common misconception about fish not needing oxygen, explaining that most fish use gills to extract oxygen from water. The paragraph emphasizes the importance of oxygen for various organisms, including humans and plants, and delves into the cellular process of respiration. It explains how cells use oxygen to produce ATP, a crucial molecule for cellular energy, through a process called cellular respiration. The video also touches on the misconception that plants do not need oxygen, highlighting that they require it for cellular respiration despite producing oxygen through photosynthesis.

05:00

🍞 Fermentation: Life Without Oxygen

The second paragraph explores what happens when oxygen is scarce, focusing on fermentation as a process that allows cells to produce ATP without oxygen. It discusses two types of fermentation: alcoholic fermentation, which is used by yeasts to produce ethanol and carbon dioxide, and lactic acid fermentation, which occurs in muscle cells during intense exercise and is also used in the production of yogurt. The paragraph explains how fermentation regenerates NAD+, an essential coenzyme for glycolysis, by using pyruvate or its derivatives as electron acceptors. It also mentions the role of ATP in cellular processes and how fermentation, while effective, is less efficient than aerobic respiration in ATP production.

Mindmap

Keywords

💡Oxygen

Oxygen is a chemical element essential for the survival of most organisms, including humans, fish, and plants. In the context of the video, oxygen plays a crucial role in cellular respiration, a process where cells use oxygen to convert glucose into ATP, the energy currency of the cell. The video emphasizes the importance of oxygen by discussing how misconceptions about its necessity for plants are incorrect, as they too require oxygen for cellular respiration.

💡Gills

Gills are specialized respiratory organs found in fish and some other aquatic animals that allow them to extract oxygen from water. The video script mentions gills as part of the childhood misconception about fish not needing oxygen, which is later corrected by explaining the function of gills in fish respiration.

💡Cellular Respiration

Cellular respiration is a set of metabolic reactions that take place in cells to convert biochemical energy from nutrients into adenosine triphosphate (ATP), and then release waste products. The video explains that this process requires oxygen as a reactant to break down glucose and form ATP, highlighting its importance in energy production within cells.

💡ATP (Adenosine Triphosphate)

ATP, or adenosine triphosphate, is a molecule that stores and transfers chemical energy within cells. The video describes ATP as 'action packed with three phosphates' and explains that it is central to powering many cellular processes. It also discusses the conversion of ATP to ADP (adenosine diphosphate) and back to ATP during cellular respiration.

💡Aerobic

Aerobic refers to processes that occur in the presence of oxygen. The video uses this term to describe a type of cellular respiration that requires oxygen, contrasting it with anaerobic processes that can occur without oxygen. Aerobic respiration is highlighted as a more efficient way to produce ATP compared to anaerobic processes.

💡Anaerobic Respiration

Anaerobic respiration is a type of cellular respiration that does not require oxygen. The video explains that certain organisms, like some bacteria and archaea, can perform anaerobic respiration using alternative electron acceptors when oxygen is not available.

💡Fermentation

Fermentation is an anaerobic process that some cells, such as yeast and muscle cells, use to produce ATP in the absence of oxygen. The video details two types of fermentation: alcoholic fermentation, which produces ethanol and carbon dioxide, and lactic acid fermentation, which produces lactate. These processes are essential for cells to continue generating energy when oxygen is scarce.

💡Glycolysis

Glycolysis is a metabolic pathway that converts glucose into pyruvate, producing a small amount of ATP and the electron carrier NADH. The video describes glycolysis as the starting point for both aerobic and anaerobic respiration, with the latter processes adding steps to regenerate NAD+ for continued glycolysis in the absence of oxygen.

💡NADH and NAD+

NADH (nicotinamide adenine dinucleotide) and NAD+ are coenzymes involved in redox reactions, essential for transferring electrons during cellular respiration. The video explains the role of NADH and NAD+ in glycolysis and how their interconversion is crucial for the regeneration of NAD+ in fermentation processes.

💡Electron Transport Chain

The electron transport chain is a series of protein complexes that use the energy from electrons to pump protons across a membrane, creating a proton gradient used to produce ATP. The video mentions that in the absence of oxygen, certain organisms use alternative electron acceptors in anaerobic respiration, bypassing the electron transport chain.

💡Lactic Acid Fermentation

Lactic acid fermentation is a type of anaerobic respiration used by muscle cells during intense exercise when oxygen supply is insufficient. The video explains that this process regenerates NAD+ by converting pyruvate into lactate, allowing glycolysis to continue and providing energy for the cell.

Highlights

Swimming was a childhood passion, starting at a young age.

The misconception that fish don't need oxygen is clarified.

Most fish use gills to extract oxygen from water.

The importance of oxygen for various organisms is emphasized.

Plants require oxygen despite producing it through photosynthesis.

The necessity of oxygen for cellular respiration is explained.

ATP, or adenosine triphosphate, is the primary energy currency in cells.

The conversion between ATP and ADP is crucial for cellular energy.

Aerobic cellular respiration is a complex process requiring oxygen.

The video focuses on cellular processes in the absence of oxygen.

Some bacteria and archaea can perform anaerobic respiration.

Yeast and muscle cells can handle low oxygen environments.

Fermentation is a process that allows ATP production without oxygen.

Glycolysis is a key step in both aerobic and anaerobic processes.

Alcoholic fermentation by yeast produces ethanol and carbon dioxide.

Lactic acid fermentation is used by muscle cells during oxygen debt.

Lactic acid fermentation's role in yogurt production is mentioned.

Fermentation's limitation in ATP production compared to aerobic respiration.

Transcripts

play00:00

Captions are on! Click CC at bottom right to turn off.

play00:06

When I was a kid, swimming was one of my favorite things to do.

play00:10

I was on the swim team starting when I was four years old and then on and off throughout

play00:14

grade school, although I wasn’t especially fast.

play00:17

I just loved the water.

play00:18

I used to dream that there was a way I could be like a fish.

play00:22

When I was little, I had a misconception that fish didn’t need oxygen.

play00:26

Later on, I learned that, no, most fish have gills that allow them to extract the oxygen,

play00:31

which they need, from the water.

play00:32

So then I just thought it’d be really cool if I just had gills.

play00:36

But, alas, no gills for Pinky.

play00:39

Oxygen is a really big deal---so many organisms---from fish to plants to humans---need oxygen.

play00:45

And yes, even though plants make oxygen in photosynthesis…they still perform cellular

play00:50

respiration and therefore plants still need oxygen themselves.

play00:53

There’s often a misconception that plants don’t need oxygen; that’s just not true.

play00:58

So why do these organisms need oxygen?

play01:00

It’s similar to why you need oxygen.

play01:02

If you’ve ever wondered why you need to breathe, which is done by the respiratory

play01:06

system in your body, zoom into the cell level.

play01:09

Cells in your body use the oxygen you inhale to perform cellular respiration.

play01:14

The formula here requires inputs, otherwise known as reactants, to make ATP.

play01:18

And oxygen is one of those reactants in the overall equation that is needed to break glucose

play01:23

down in forming ATP.

play01:26

Why ATP?

play01:27

ATP stands for adenosine triphosphate.

play01:29

It is action packed with three phosphates.

play01:33

It has the ability to power many cellular processes.

play01:36

Typically it’s coupled to other things that it may be powering.

play01:40

Now after losing the phosphate, the molecule is ADP, adenosine diphosphate, because it

play01:46

has 2 phosphates.

play01:47

In cellular respiration though, there are enzymes that can add another phosphate to

play01:51

it and convert it back into ATP again.

play01:54

This particular formula of cellular respiration is aerobic, meaning overall, it requires oxygen.

play02:00

It is pretty complex, and we have a video breaking down steps.

play02:03

But that’s not what this video is about.

play02:06

This video is about what happens when there is no oxygen.

play02:10

Because cells still need to make their ATP.

play02:13

So what kind of cells can handle the no oxygen thing?

play02:17

Well many types of bacteria can.

play02:19

Many types of archaea.

play02:20

Yeast, which is a fungus that could be helpful like making your bread rise.

play02:24

Your muscle cells can, for a while anyway.

play02:27

These are all just some examples.

play02:29

Now these organisms handle the lack of oxygen in different ways.

play02:33

Some organisms such as some types of bacteria or archaea can do anaerobic respiration---

play02:40

they can continue to perform glycolysis, krebs, and the electron transport chain just like

play02:45

aerobic cellular respiration.

play02:47

But since there is no oxygen to be that final electron acceptor at the end of the electron

play02:52

transport chain, they use something else.

play02:55

Sulfate for example.

play02:57

These organisms are specifically adapted to be able to use a different electron acceptor

play03:01

in this anaerobic respiration.

play03:04

Another option is the organism may just stick with doing just glycolysis, which doesn’t

play03:10

require oxygen, and then the addition of some way to get their NAD+ back----we’ll talk

play03:16

about what that means in a minute.

play03:18

This process is called fermentation and that’s what we’re going to focus on.

play03:23

Fermentation is a way to be able to handle the little to no oxygen issue: it allows for

play03:28

glycolysis to happen and for glycolysis to keep going.

play03:33

That means making ATP when there is no oxygen.

play03:36

And while you won’t make as much ATP in this process as you would aerobic cellular

play03:40

respiration, you can’t be too picky when oxygen isn’t around.

play03:44

Recall what glycolysis is from our cellular respiration video: in glycolysis, you take

play03:50

glucose---a sugar---and it gets converted into pyruvate.

play03:54

This takes a little ATP cost to actually start it up, but overall, you make 2 net ATP per

play04:00

glucose molecule and you also produce 2 NADH.

play04:03

What’s that?

play04:04

Recall that NADH is a coenzyme and an electron carrier.

play04:09

We also need to mention that NADH didn’t just *poof* appear as a product.

play04:13

No, because NAD+ actually was reduced to NADH when it gained electrons.

play04:18

And if the words reduced and oxidized are confusing…you can remember the famous LEO

play04:24

GER mnemonic: Lose electrons= oxidized.

play04:28

Gained electrons=reduced.

play04:30

So NADHNAD+ is oxidation because it loses electrons and NAD+NADH is reduction because

play04:39

it gains electrons.

play04:41

Now NADH, the electron carrier, would normally be delivering the electrons gained to the

play04:47

electron transport chain if this was aerobic cellular respiration.

play04:52

Once losing their electrons, NADH would be oxidized into NAD+ and be ready to be involved

play04:57

all over again in glycolysis.

play05:00

But there’s no electron transport chain step in this fermentation process.

play05:03

So we’ve got to regenerate the NAD+ somehow—NAD+ is needed here after all for glycolysis to

play05:10

continue.

play05:12

Fermentation therefore adds another little step to the end of glycolysis---a step to

play05:16

help regenerate NAD+.

play05:19

This happens because fermentation allows NADH to give its electrons to an electron acceptor

play05:24

which, in the two fermentation examples we are going to give, will either be a derivative

play05:29

of pyruvate or pyruvate itself.

play05:32

So here we go with two types of fermentation which both result in different products from

play05:38

pyruvate.

play05:40

Alcoholic fermentation: as done by some types of yeast.

play05:43

So first glycolysis which yields 2 net ATP, 2 pyruvate, and 2 NADH.

play05:49

Now we need the step to regenerate the NAD+ so we can keep doing glycolysis.

play05:55

The 2 pyruvate is used which will ultimately produce carbon dioxide and 2 ethanol (alcohol),

play06:02

but the derivative of pyruvate shown here, acetaldehyde, can act as an electron acceptor

play06:06

in this process so that the 2 NADH can be oxidized to 2 NAD+ so that glycolysis can

play06:13

start all over.

play06:14

Since ethanol (alcohol) is a waste product in this process.Yeasts also can do alcoholic

play06:22

fermentation in making bread, and the carbon dioxide product we mentioned is involved with

play06:27

helping the bread rise!

play06:29

The tiny amount of alcohol produced in the short fermenting time of bread will evaporate

play06:34

in the baking process.

play06:36

Lactic acid fermentation: as can be done by cells such as your muscle cells for example!

play06:40

While your muscle cells can do aerobic cellular respiration, they can shift to lactic acid

play06:45

fermentation if they experience an oxygen debt.

play06:49

This could happen if you are working out very intensely where your blood is unable to deliver

play06:53

a sufficient amount of oxygen to them for their demand.

play06:57

Just like with alcoholic fermentation, we start with glycolysis that yields 2 net ATP,

play07:02

2 pyruvate, and 2 NADH.

play07:06

But now we need the step to regenerate the NAD+, and this step is a bit different from

play07:11

alcoholic fermentation.

play07:13

The 2 pyruvate on the reactant side will ultimately yield 2 lactate.

play07:18

The pyruvate can act as an electron acceptor allowing NADH to be oxidized to NAD+ so that

play07:25

glycolysis can start over.

play07:27

By the way, this lactate product or specifically its other form lactic acid, has often been

play07:33

blamed for the muscle soreness that occurs the day after intense exercise- in many of

play07:38

my teaching years this was the hypothesis with this- but actually there’s some recent

play07:41

research that may dispute this product as the cause of muscle soreness.

play07:45

Check out our further reading suggestions in our video details to learn more!

play07:49

Lactic acid fermentation is also done by bacteria that are involved in making yogurt and lactic

play07:54

acid can contribute to its sour taste.

play07:57

So overall, fermentation is a pretty remarkable process.

play08:02

Although, it does make us appreciate oxygen because despite how absolutely awesome fermentation

play08:08

may be….…it just can’t make as much ATP as aerobic cellular respiration.

play08:12

Well, that’s it for the Amoeba Sisters and we remind you to stay curious!

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الوسوم ذات الصلة
Cellular RespirationFermentationOxygen NeedATP ProductionAnaerobic ProcessesYeast FermentationMuscle CellsLactic AcidEthanol ProductionCellular Energy
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