Entry to the automatic Titration - TitroLine 5000

Summary

This video provides a detailed walkthrough of using the Titrator Line 5000, focusing on its setup, calibration, and application for automatic titrations in food and beverage testing. It covers the process of connecting electrodes, calibrating pH, preparing reagents, and performing titrations for total acidity in juices, like orange juice. The video emphasizes the ease of use, with step-by-step instructions on selecting standard methods, conducting measurements, and printing results. It's an ideal guide for users looking to streamline their titration processes with automated solutions.

Takeaways

• 😀 The Titration System 5000 is ideal for determining total acidity in juices and other food products like mayonnaise, ketchup, and mustard.
• 😀 The system allows for easy setup and operation, including automatic calibration and reagent filling.
• 😀 The pH electrode calibration is performed using two-point calibration with pH 4 and pH 7 buffer solutions.
• 😀 A magnetic stirrer, connected via gold contacts, is used to agitate samples during titration for uniform results.
• 😀 Standard methods for various applications are pre-programmed, and you can select the desired method directly from the interface.
• 😀 The Titration System provides real-time titration curves on a graphic display, showing the titration process and current pH/volume.
• 😀 Results are automatically calculated and can be printed or saved in PDF/CSV format on a USB stick.
• 😀 The system supports integration with external devices like a USB keyboard and balance for sample ID entry and weight transfer.
• 😀 The reagent setup is simplified with a dedicated adapter for reagent bottles, and protective measures are in place (e.g., soda lime for CO2 protection).
• 😀 Once calibration is complete, the system is ready for sample measurement, and results are automatically displayed after the titration process finishes.
• 😀 The system is designed for ease of use, and even new users can perform titrations with minimal training by following step-by-step prompts.

Q & A

What is the primary function of the TiterLine 5000 described in the video?

The TiterLine 5000 is an automatic titration instrument designed to determine the total acidity in liquids like fruit juices, mayonnaise, ketchup, mustard, and other food products. It can also be used for applications such as determining chloride content in food or water.

What are the main components included in the scope of delivery for the TiterLine 5000?

The scope of delivery includes a magnetic stirrer, hand controller for manual titrations, beakers for pH electrode calibration, a titration clamp, pH electrode stand, a vessel for saturation solution, buffer solutions for calibration, USB cable, power supply, adapter, reagent bottles, and the TiterLine 5000 itself.

How do you connect the TiterLine 5000's components during setup?

First, you connect the magnetic stirrer to the instrument via gold contacts. Then, the titration tip is attached to the titration clamp, and the pH electrode, along with the temperature sensor, is connected. Reagent bottles are also connected via tubing, and an optional USB hub, keyboard, and memory stick can be attached for easier sample management.

What reagent is used for the titration of total acidity in fruit juices?

For the titration of total acidity in fruit juices, sodium hydroxide is used as the titration reagent. Soda lime is also required for CO2 protection when using sodium hydroxide.

What is the purpose of the pH electrode calibration, and how is it done?

The pH electrode calibration ensures accurate pH measurements during titration. The calibration is done using two buffer solutions (pH 4 and pH 7). The electrode is rinsed, immersed in each buffer, and calibrated using the TiterLine's calibration menu.

How is the sample prepared for titration in the video?

In the example shown, 10 milliliters of orange juice are measured and then diluted with 60 milliliters of deionized water before being placed in the beaker for titration.

What is the role of the online curve displayed during titration?

The online curve during titration visually shows the progress of the titration, displaying the change in pH as the titration reagent is added. It helps monitor the reaction and determine when the endpoint is reached.

What happens when the titration reaches the endpoint?

When the endpoint is reached, the TiterLine 5000 automatically calculates the result of the titration and displays it on the screen. The result can also be printed as a PDF or saved to a USB stick.

How do you ensure that the system is ready for the next titration?

After each titration, the titration tip and other components are rinsed thoroughly. This ensures that the system is clean and ready for the next measurement.

What makes the TiterLine 5000 easy to use for multiple applications?

The TiterLine 5000 includes a set of default methods for various applications, such as measuring total acidity or chloride content. These pre-programmed methods can be easily selected and used for a wide range of titration tasks, making the instrument versatile and user-friendly.