PSPA Blok IV - 03 KLromatografi Lapis Tipis

Summary

In this video, Rafli, a pharmacist, demonstrates a practical simulation for testing the chromatographic profile of guava leaf extract. The video covers four key competencies: data collection, chromatography simulation, report generation, and professional conduct. Rafli guides viewers through the steps of sample preparation, chromatography plate handling, and elution, including detailed instructions on calculating movement phase composition and RF values. Emphasizing professionalism, the video also showcases the importance of lab attire and proper handling of materials. This practical approach is aimed at reinforcing the skills necessary for quality control in traditional pharmaceutical testing.

Takeaways

• 😀 The video introduces a pharmacist explaining the simulation of chromatographic testing for guava leaf extract in a practical setting.
• 😀 The simulation involves four main competency areas: data collection, practical simulation, recording and reporting, and professional behavior.
• 😀 Competency area 1 emphasizes gathering data from references, including herbal pharmacopoeias, and identifying relevant information like mobile and stationary phases, solvent concentrations, and sample volumes.
• 😀 In competency area 2, the simulation demonstrates practical steps in thin-layer chromatography, including sample preparation, chamber saturation, and applying the mobile phase.
• 😀 The importance of professional attire and safety protocols, such as wearing lab coats, masks, and gloves, is emphasized throughout the procedure.
• 😀 Proper time management is essential during the experiment, including the careful process of saturating the chamber and handling the samples with precision.
• 😀 The process of applying samples onto the chromatography plate includes pre-treatment with filter paper to ensure effective absorption of the sample.
• 😀 The chromatographic plates are placed into the chamber for elution, where the sample will separate based on its affinity to the mobile and stationary phases.
• 😀 After elution, the plates are dried, and identification is performed using UV light to observe the separation of the sample's components.
• 😀 Competency area 3 focuses on calculating the mobile phase composition and RF values for the separated sample spots, helping to determine the sample's chemical profile.
• 😀 Competency area 4 covers the importance of professional conduct, including appropriate attire, maintaining a clean workspace, and accurately reporting results without errors or corrections.

Q & A

What is the main objective of the simulation described in the script?

The main objective of the simulation is to demonstrate the chromatographic profiling of guava leaf extract, focusing on its identification, data collection, and professional conduct in a pharmaceutical industry setting.

What are the four key competencies outlined in the script for the chromatographic testing?

The four key competencies are: 1) Data collection and information gathering, 2) Practical simulation of chromatographic identification, 3) Recording and reporting on mobile phase calculation and RF value, and 4) Demonstrating professional attitude and behavior.

What is the significance of using the 'Farmakope Herbal Indonesia' during the simulation?

The 'Farmakope Herbal Indonesia' serves as a reference material for the simulation, providing essential monograph data on the guava leaf extract needed for the chromatographic testing.

How is the mobile phase for the chromatography prepared, and what solvents are used?

The mobile phase is prepared by combining chloroform, acetone, and formic acid in a ratio of 10:2:1, respectively. The total volume for the mobile phase is 100 ml.

Why is the chamber pre-saturated before starting the chromatography?

The chamber is pre-saturated to ensure that the mobile phase is fully saturated in the environment, which is essential for accurate chromatography results.

How many times should each sample be spotted on the chromatographic plate, and why?

Each sample should be spotted at least three times to ensure proper absorption and distribution of the sample on the chromatographic plate, leading to more reliable results.

What is the purpose of using UV light for identifying the chromatographic profile?

UV light is used to identify and visualize the chromatographic profile by detecting the presence of specific compounds based on their UV absorption properties.

How is the RF value calculated, and why is it important in this simulation?

The RF value is calculated by dividing the distance traveled by the sample spot by the distance traveled by the solvent front. It is important because it helps in comparing the mobility of the compounds in the sample and determining their identity.

What should be done if the chromatographic plate has not been activated, according to the script?

If the chromatographic plate has not been activated, it must be heated in an oven before it can be used for spotting the samples.

What is the role of professional conduct during the simulation, as described in the script?

Professional conduct is essential throughout the simulation, which includes wearing the proper lab attire, using safety equipment like masks and gloves, handling materials with care, and maintaining a tidy workspace to ensure safety and accuracy in the results.