Analisis Kecernaan In Sacco - Teknik Laboratorium dan Penelitian Nutrisi Makanan Ternak

Summary

This video script explains the insako method used to estimate the degradation rate of feed materials in livestock rumen. The process involves preparing nylon bags, incubating feed samples in the rumen of fistulated animals, and analyzing the remaining feed after specific time intervals. The method offers advantages like practical and efficient measurement of degradation kinetics, cost-effectiveness, and the ability to analyze multiple feed types simultaneously. It also covers the preparation of samples, nylon bags, and details on factors influencing degradation such as particle size and incubation time, providing a comprehensive guide for laboratory practice in animal nutrition research.

Takeaways

• 😀 The 'in sacco' method estimates feed degradation and its rate by rumen microbes.
• 😀 The nylon bag is used to contain the feed sample, which is incubated in the rumen of a fistulated animal.
• 😀 Key factors affecting degradation include feed type, particle size, nylon bag porosity, incubation time, and washing consistency.
• 😀 Nylon bags are pre-treated by sealing all sides except the top, which is left open for sample insertion.
• 😀 Samples are weighed and sealed into the nylon bags, which are then incubated for set periods ranging from 4 to 96 hours.
• 😀 After incubation, the bags are washed with flowing water until the wash water is clear, then dried to stabilize their weight.
• 😀 The degradation rate is calculated by measuring the loss of sample weight after washing and drying.
• 😀 The method is simple, cost-effective, and efficient, as it focuses specifically on rumen conditions.
• 😀 One of the key advantages of this method is its ability to create degradation kinetics graphs over time.
• 😀 While 'in sacco' is practical and informative, it has limitations, such as uncertainty about the exact point of optimal degradation.

Q & A

What is the purpose of the in sacco method in animal nutrition research?

The in sacco method is used to estimate the degradation rate and degradation of feed by rumen microbes in livestock. The method involves placing feed samples in nylon bags, incubating them in the rumen of fistulated animals, and measuring the loss in weight as an indicator of microbial degradation.

How are the nylon bags prepared for the in sacco method?

The nylon bags are cut to a size of 11 cm by 16 cm, with some extra length. The edges are sealed using Schiller glue, leaving the top open for sample insertion. The bags are then oven-dried at 50°C and weighed before use.

Why is it important to use nylon bags with specific porosity for the in sacco method?

Nylon bags used in the in sacco method typically have a porosity of 40-60 microns. This size ensures that rumen microbes can enter the bags to degrade the feed while preventing the feed from leaking out, providing accurate degradation results.

What factors influence the degradation rate in the in sacco method?

The degradation rate is influenced by factors such as the type and particle size of the feed, the porosity of the nylon bag, the duration of incubation in the rumen, and the uniformity of the washing procedure after sample removal.

What is the role of the adaptation phase in the in sacco method?

The adaptation phase helps acclimate the animals to the feed sample being tested and prepares the rumen microbes to properly process the new feed material. It also ensures the removal of any residual feed from previous trials.

How are feed samples prepared before being placed into the nylon bags for incubation?

Feed samples are first ground to a size of 2 mm using a Willem mill. They are then oven-dried at 50°C until their weight stabilizes, ensuring that the moisture content is consistent before being placed in the nylon bags.

What is the purpose of washing the nylon bags after the incubation period?

The washing of the nylon bags ensures that all the residual feed material is removed. The bags are washed in running water until the water runs clear, ensuring that any microbial degradation products are properly cleaned away.

How does the in sacco method compare to in vivo methods of studying feed degradation?

The in sacco method is more practical and efficient compared to in vivo methods. It is less time-consuming and cost-effective, focusing on the rumen environment directly, while in vivo methods involve more complex animal management.

What are the advantages of using the in sacco method for feed degradation analysis?

The advantages include its ability to generate degradation kinetics, its simplicity, lower cost, and ability to analyze multiple feed samples simultaneously. It is also quicker than in vivo methods, allowing researchers to obtain results faster.

What are the limitations of the in sacco method?

The limitations of the in sacco method include the inability to determine the exact point of optimal feed degradation and the potential for variability in microbial activity during the incubation period. Researchers often rely on literature values for estimated degradation times.