Memindahkan Porang Kultur Jaringan ke Tanah - AKLIMATISASI (Episode 5/end)

Summary

The video explains the critical process of acclimatizing lab-grown plants to outdoor conditions. It details each step, from removing plants from cultivation containers and washing them to prevent contamination, to treating them with fungicide. The plants are then transplanted into a new growing medium and covered to maintain moisture. Over the next month, the plants are gradually introduced to external conditions, monitored for growth, and once fully adapted, they are moved to larger spaces or the ground. This process ensures the plants transition smoothly and thrive in their new environment.

Takeaways

• 😀 The acclimatization process is critical when transitioning plants from an in vitro (lab) environment to an ex vitro (field) environment.
• 🌱 The first step in acclimatization is ensuring the plant has well-developed stems and roots.
• 💧 Acclimatization involves adjusting plants to higher temperatures and lower humidity, which can be stressful for them.
• 🚫 A fungicide solution is used to treat the plant for 5 minutes to prevent fungal and pathogen growth.
• 🌿 After cleaning the plant, it is transferred into a soil and manure mixture to encourage proper root development.
• 🧴 Maintaining high humidity around the plant during the initial stage of acclimatization is essential to prevent shock.
• ⏳ A plastic cover or cup is placed over the plant to maintain humidity for around a month while it adjusts to the new environment.
• 🔄 The plant’s adaptation is monitored by gradually opening the cover and assessing its growth after one month.
• 🌞 If the plant shows signs of wilting or poor growth, it is placed back under the cover until it can adapt fully.
• 🌍 After 3-4 months, the plant is strong enough and ready for final transplanting into the field or outdoor environment.

Q & A

What is the acclimatization process for laboratory-grown plants?

The acclimatization process is the transition of plants from an in-vitro (laboratory) environment to an ex-vitro (outdoor) environment. This involves gradually adjusting the plants to changes in temperature, humidity, and environmental conditions.

Why is the acclimatization process critical for plant growth?

It is critical because sudden changes in temperature and humidity can cause the plants to 'crash', meaning they will not survive or grow well in their new environment.

What is the first step in the acclimatization process after the plants are ready?

The first step is to open the bottles containing the plants. This allows them to begin adjusting to the external environment and reduces the risk of sudden environmental shock.

Why is it important to wash the plants after removing them from the bottles?

Washing the plants removes residual agar and nutrients from the bottles, ensuring that no remnants interfere with the plant's transition to its new growth medium.

What is the purpose of soaking the plants in a fungicide solution?

The fungicide solution is used to treat the plants for any fungi or pathogens that may have developed in the lab environment, ensuring they are disease-free before being transferred to their new environment.

What is the recommended fungicide dosage for treating the plants?

The recommended dosage is 2 grams of fungicide per liter of water. The plants should be soaked in this solution for about 5 minutes.

What type of growth medium is used after the plants are washed and treated?

The plants are planted in a growth medium made from a mixture of soil and manure in a 1:1 ratio. This provides the plants with the nutrients they need for further development.

Why are plastic cups used in the acclimatization process?

Plastic cups are used to maintain humidity around the plants, helping them avoid stress during the early stages of acclimatization by keeping the environment moist.

How long does the initial acclimatization period last, and what is monitored during this time?

The initial acclimatization period lasts for about one month. During this time, the plants' root development and their ability to adapt to their new environment are closely monitored.

What happens after one month of acclimatization?

After one month, the plastic covers are gradually removed to expose the plants to more external conditions. The plants are monitored to ensure they are not wilting or showing signs of stress.

What signs indicate that the plants are ready for transplantation?

If, after removing the plastic covers, the plants remain upright and healthy, with strong growth, they are considered ready for transplantation into the open ground.

What is the final step after the plants have successfully acclimatized?

The final step is to transplant the plants into the soil. They should now have well-formed stems and roots, and be strong enough to thrive in their new environment.

What ongoing care is required after transplantation?

After transplantation, the plants need ongoing care, including regular watering to maintain soil moisture. This is especially important during the first few months after planting.