DNA Fingerprinting

Bozeman Science

11 May 201206:10

Summary

TLDRIn this podcast, Mr. Andersen explains DNA fingerprinting, also known as DNA profiling, a method developed by Alec Jeffries in 1984. The technique identifies individuals by analyzing unique variations in their DNA, particularly using short tandem repeats (STRs). DNA fingerprinting has applications in forensics and paternity testing. Through processes like gel electrophoresis, scientists can distinguish individuals based on DNA patterns. While this technique has been crucial, it's gradually being replaced by full DNA sequencing. Mr. Andersen also raises concerns about privacy and potential misuse of genetic information in databases.

Takeaways

🧬 DNA fingerprinting, also known as DNA profiling or genetic fingerprinting, was pioneered by Alec Jeffries in 1984.
🔬 DNA fingerprinting was initially developed at the University of Leicester, and all testing went through this university for the first three years.
📊 The majority of human DNA is identical (99.9%), but variability exists in the non-coding regions like STRs (short tandem repeats), which are used in DNA profiling.
🧪 Short tandem repeats (STRs) are sequences of DNA that repeat multiple times, and these vary between individuals, making them useful for identification.
🔖 DNA fingerprinting involves cutting out sections of DNA, amplifying them through polymerase chain reaction (PCR), and separating them using gel electrophoresis.
⚖️ DNA fingerprinting is used in forensics and paternity testing to match DNA samples with suspects or to determine parentage.
🚨 In forensic cases, DNA profiling is used to compare blood or other biological samples left at a crime scene with suspects' DNA.
👥 The method examines multiple DNA sections (typically 13 highly variable areas) to ensure a reliable match between individuals.
🔄 Though DNA fingerprinting is reliable, it is being gradually replaced by full DNA sequencing, which provides even more detailed genetic information.
⚠️ The FBI maintains a DNA database in the US, but concerns are raised about genetic privacy, as DNA can reveal predispositions to certain diseases like Alzheimer's or cancer.

Q & A

What is DNA fingerprinting also known as?
-DNA fingerprinting is also known as DNA profiling or a genetic fingerprint.
Who is credited with developing the concept of DNA fingerprinting?
-Alec Jeffries, working at the University of Leicester, is credited with developing DNA fingerprinting around 1984.
What did Alec Jeffries discover about DNA through his work?
-Alec Jeffries discovered that by examining certain patterns in DNA, he could determine relationships between individuals, such as paternity.
What percentage of human DNA is identical across all individuals?
-99.9% of human DNA is identical across all individuals, but variations in certain regions, like alleles, make each person unique.
What are short tandem repeats (STRs), and why are they important in DNA fingerprinting?
-STRs are sequences of DNA that repeat multiple times. They vary greatly among individuals and are used in DNA fingerprinting to distinguish between people.
What method is used to separate and visualize DNA fragments in DNA fingerprinting?
-DNA fragments are separated and visualized using gel electrophoresis, where DNA, being negatively charged, moves towards a positive charge through a gel.
Why is DNA fingerprinting considered a useful tool in forensics and paternity cases?
-DNA fingerprinting can determine who left biological evidence at a crime scene or establish paternity by comparing specific DNA sequences between individuals.
How accurate is DNA fingerprinting in identifying individuals?
-The odds of two people having the same DNA fingerprint are astronomically low, except in the case of identical twins.
Why might DNA fingerprinting be replaced by DNA sequencing in the future?
-DNA sequencing provides a more complete and detailed analysis of an individual's DNA, making it a more precise method compared to DNA fingerprinting.
What concerns are raised regarding the storage of DNA in databases?
-There are concerns that stored DNA could be used for purposes like determining predispositions to diseases, which could lead to privacy issues or misuse by insurance companies.