The Science of Color using pH, Pigments and Photos (Diehl & Girtain)
Summary
TLDRChristine Gertin and Nathan from New Jersey and Maryland present a science unit on the science of color using pH and pigment. They discuss materials, objectives, and career connections, highlighting Dr. Rabie Jabor's work on a rapid cyanide detection test. The unit includes hands-on activities like chromatography with leaves, using an RGB colorimeter app, and gel electrophoresis with dyes. Students explore color perception in nature, engage with STEM careers, and present their findings in a gallery walk. The lesson integrates technology, practical experiments, and critical thinking, making science engaging and relevant.
Takeaways
- 🌈 Christine and Nathan are presenting a unit on the 'Science of Color', focusing on the use of pH, pigments, and photos.
- 📝 They have a list of materials for the audience to review on their own, including a standards lesson objective and career connection information.
- 🔬 Dr. Rabie Jabor's work at DEVCOM in Aberdeen Proving Grounds, Maryland, is highlighted, specifically his development of a rapid cyanide detection test for triage in cases like wildfires.
- 🌿 The unit includes STEM career exploration and a discussion on how nanostructure color and magnetic charge are used in nature, with examples from bees recognizing flower colors under UV light.
- 🦋 The script mentions the use of videos to engage students, such as one explaining how butterflies and birds perceive blue color differently due to nanostructures.
- 🎨 For hands-on activities, students will use chromatography to extract pigments from leaves using isopropanol and analyze the separation on paper.
- 📲 Students will use the Carolina RGB Colorimeter app to measure RGB values of pH test strips, creating a standard table and predicting pH values of unknown samples.
- 📊 High school students will go further by using a spreadsheet to calculate a line of best fit and an equation to predict pH values more accurately.
- 🖌️ The explanation portion of the unit involves graphing values from chromatography and understanding color perception through reflection and absorption.
- 🧬 Introduction to gel electrophoresis is provided, explaining how to use micropipettes and the polarity and charge of molecules in a gel.
- 📊 Evaluation involves a gallery walk where students present their lab results in a scientific poster format, receiving feedback from peers.
Q & A
Who are the presenters in the video script?
-Christine Gertin from New Jersey and Nathan from Maryland are the presenters.
What is the main focus of the unit they are discussing?
-The main focus of the unit is the science of color, using pH and pigment, and how these are studied through photos.
What is Dr. Rabie Jabor's current project about?
-Dr. Rabie Jabor is working on a rapid test that detects cyanide in patients for triage, especially useful in cases like wildfire where cyanide compounds can be released.
What is the purpose of the rapid cyanide test developed by Dr. Rabie Jabor's team?
-The purpose of the rapid cyanide test is to determine the level of exposure to cyanide compounds in cases like wildfire, allowing for faster medical treatment and aid.
How do the students explore the use of color in nature using chromatography?
-The students explore the use of color in nature by investigating how scientists determine what color pigments contribute to the colors we see, including examining the role of nanostructure color and magnetic charge.
Why do bees see flowers differently than humans?
-Bees see in the ultraviolet spectrum, which is not perceptible to human eyes, allowing them to perceive patterns and colors on flowers that are invisible to us.
What is the significance of the Carolina RGB Colorimeter app in the activity?
-The Carolina RGB Colorimeter app is used to measure the RGB values of colors, allowing students to quantify color and create a standard table for pH strips, which they can then use to predict the pH of unknown samples.
How do high school students take the activity to the next level using a spreadsheet program?
-High school students use a spreadsheet program to calculate a line of best fit and an equation of a line, which they use to predict the pH value of unknown samples based on the RGB values from the pH test strip.
What is the purpose of the gel electrophoresis activity in the molecular rainbow lab?
-The gel electrophoresis activity allows students to investigate the polarity and size of different dyes and molecules, and how they separate within a gel based on their charges.
How do students present their findings in the evaluation portion of the unit?
-Students present their findings through a gallery walk, using Google Slides or traditional chart paper to create scientific poster presentations, and receive feedback from their peers.
What is the role of the teacher in the molecular rainbow lab gel electrophoresis activity?
-The teacher provides various dyes for the students to use in the gel electrophoresis activity and sets up the gel so that the wells are in the center, allowing for the investigation of molecular polarity.
Outlines
🌈 Science of Color Exploration
Christine and Nathan introduce a science unit focused on the science of color, utilizing pH, pigments, and photography. They present a list of materials and a lesson objective, touching on a career connection with Dr. Rabie Jabor's work at DEVCOM in Maryland, developing a rapid cyanide detection test for medical triage post-wildfire scenarios. The unit also covers STEM careers and engages students in exploring nanostructures, color, and magnetic charge in nature using chromatography. It discusses bees' unique ultraviolet vision and how it aids in targeting flowers, as well as the scarcity of blue pigments in nature due to nanostructures. Teachers are provided with background concepts and activities, such as paper chromatography with Skittles or M&M's, and students extract pigments from leaves using isopropyl alcohol. The lesson includes hands-on activities with chromatography paper and the use of the Carolina RGB Colorimeter app for a quantitative approach to color analysis.
📈 Advanced Color and pH Analysis
The script progresses to the high school level, where students delve deeper into color and pH analysis. They standardize a pH-RGB table using the RGB app and apply it to predict pH values of unknown samples, such as rainwater, to understand the impact of acid rain. The explanation section involves chromatography of black ink to understand color separation and perception, with students graphing values and predicting color positions based on wavelengths. The script introduces gel electrophoresis, explaining how to use micropipettes and setting up a microplate header. Students investigate the polarity and charge of molecules, observing how positive and negative molecules move in opposite directions within the gel. The evaluation involves a gallery walk, where students present their findings from chromatography and electrophoresis in the form of scientific posters, receiving feedback via sticky notes to improve their presentations before final submission.
📚 Comprehensive Lab and Activity Overview
The final paragraph provides an overview of the lab and activities, including references and handouts for teachers. It emphasizes the importance of the hands-on approach in understanding the science behind color and pH, and how technology can enhance the learning experience. The paragraph concludes with a mention of the resources available for educators to facilitate the lab activities and ensure a comprehensive understanding of the subject matter.
Mindmap
Keywords
💡Science of Color
💡pH
💡Pigment
💡Chromatography
💡Nanostructure
💡Magnetic Charge
💡Cyanide
💡RGB Colorimeter
💡Gel Electrophoresis
💡Spectrophotometry
💡STEM Careers
Highlights
Christine Gertin and Nathan from Maryland are working on a unit about the science of color, utilizing pH, pigment, and photos.
The project involves creating a rapid test to detect cyanide in patients for triage, especially in cases of wildfire exposure.
Dr. Rabie Jabor is developing a test to determine cyanide exposure levels for faster medical treatment in emergency situations.
STEM careers are highlighted, with a list provided from various websites for students to explore.
Students will explore nanostructure color and magnetic charge in nature using chromatography.
Bees recognize flower colors in the ultraviolet spectrum, which is different from human perception.
Most blue in nature is due to nanostructure and not actual pigment, as seen in the blue morpho butterfly.
Teachers are provided with background content and resources from Science Buddies for paper chromatography.
Students will extract pigment from leaves using isoprenol and analyze the results with chromatography paper.
The Carolina RGB Colorimeter app is introduced for students to quantify RGB values of colors.
Students will create a standard table for pH strips using RGB values and predict unknown pH levels.
High school students will use spreadsheet programs to calculate a line of best fit for pH prediction.
Gel electrophoresis is introduced to students to investigate the polarity and size of molecules.
Students will predict molecular charges and observe their separation within a gel based on polarity.
Evaluation involves a gallery walk where students present their findings in a scientific poster format.
Feedback is collected through sticky notes for students to improve their presentations.
References and handouts are provided for teachers to support the lab and activities.
Transcripts
all right my name is Christine gertin
I'm from New Jersey and I have been
working with Nathan de I'm from
marylands and our unit is the science of
color and using pH and pigment and
photos we have a list of materials which
we won't read through that you can read
through on your own and then our
[Music]
standards lesson
objective and then a little bit about
the Career Connection and the mentor
that Nathan has been working for
with so uh Dr rabie Jabor has been
working at um devcom over here in Aber
Proving Grounds Maryland uh running the
CBC lab which um is working on
investigating various aspects of cyanide
um and he's making a rapid test which
texts detects cyanide uh in patients for
triage so so in cases like wildfire and
a lot of compounds that are used in the
home and other industrial applications
if it gets burned in a wildfire releases
cyanide compounds and we can develop a
test to determine how much exposure they
had so that we can help them get uh Aid
faster and get medical treatment faster
um so that's what the current project
that we're working on now and that's
what this unit is based on great uh we
have a list of some stem careers as well
that list multiple care careers from
each of the websites there and then for
engagement the students will explore how
Nano structure color and magnetic charge
are used in nature using chromatography
the students will investigate how
scientists determine what color pigments
contribute to the color we see in nature
and Nathan's going to discuss the flower
and B video a little bit so to get
students hooked on uh this idea we show
how bees actually recognize flower
colors um bees see in the ultraviolet
which is not uh perceptible to human
eyes and so what looks like a pretty
flower to us looks vastly different to
beads in fact the image that you see
here is actually yellow flower with a
white center but under UV light it looks
just like a Target so bees are able to
Target it easier so we get students
excited about and curious about how
color plays a role in various aspects of
biological
life the second video that can also be
shown is butterflies and birds talking
about how the ability to see blue color
happen later in evolution and so there's
not a lot of blue pigment that occurs
naturally in nature most of the blue
that is occurring like in the blue
morpho butterfly is due to Nano
structure and reflecting the life off of
that Nano structure and not actual
pigment chemically that's doing that
refraction or reflection or
absorption um there's also some
background concept content for the
teachers to review through Science
Buddies it gives a great video on paper
chromatography and gives some options on
how they can also change what they're
doing and use different solvents and use
different things that they want to
extract pigment from gives an example of
Skittles or M&M's uh in the activity
that we chose to do we're actually
having the students extract pigment from
leaves and the students will do that
with
isoprenol which is pretty much rubbing
alcohol and they they'll be able to do
that and then put it on the
chromatography paper and look at the
separation of the
pigments in their individual leaves
which they can either pick out
themselves depending on their age group
or the teacher can supply for them so
they'll get some Hands-On background and
they'll be able to discuss what pigments
were in those actual leaves next portion
is the explanation
portion and in the exploration portion
they're going to need their cell phones
or pair them up with another student
that has a cell phone and download the
free app from the either Apple I store
or the Google Play store called Carolina
RGB
Colorimeter uh this app is basically
where you kind of point and shoot at any
object and it will give you the r GB or
red green blue values for that
particular color um in this activity
they're going to use the
color key for a pH strip and basically
figure out the RGB values for that pH
strip and kind of create a standard
table and then they're going to pick
unknown samples and you can do two
things you can either create samples of
your own of known phes and have them um
experiment with that to see how they can
uh predict the pH from the RGB value of
the test strip and compare it to the
standards or they can collect various
rainwater and other water sources from
around their areas and make that
connection of of acid rain and and rain
in the you know gutters or a pool or a
driveway where they can test the pH of
that using their activities so that's at
the middle school level at the high
school level it goes a little bit
further where they're doing the same
standardization table of the ph and the
RGB app but they're actually going to
then um use a spreadsheet program to
calculate a line of best fit and an
equation of line and use that math equ
line to predict the pH value of the
unknown samples so you would hold off on
those rainwater samples until they've
made their equation of the line and then
they'll actually try to predict the pH
using the RGB value of the the pH test
strip and see how accurate it is
compared to the actual strip um it's a
just a quick exploration of the app and
using pH paper and using it for a
particular purpose other than just you
know pH they actually get to use
technology to make their qualitative of
color more quantitative in terms of RGB
values and then for explanation uh
students are oh Nathan do you want to go
over the explanation sure uh in the
explanation they're going to use this
knowledge of chromatography and their
RGB uh program to basically they're
going to get a uh already uh a example
chromatography paper where it's
separated black ink from a pen into its
three constituent colors of blue magenta
and cyan and they're going to use a
graph to basically graph the values
based on the distance that they travel
um they're going to explain how color is
um perceived such like reflection
absorption they're going to kind of
quantify their values and they have a a
color table so they have the wavelengths
of color where they can predict where
red and green would be on that line a
best fit
and then they would also get to explain
um a little bit about the process
of um separating colors and kind of
using the thing hey there might be
another way to separate colors that's
not through chromatography so it kind of
gives you a way for them to explain what
they know so far as kind of a formative
assessment uh until they get to another
lab and then the final
um
evaluation all right and then for
elaborate we're going to introduce them
to gel electri foris if students do not
have prior knowledge on how to
micropipet there is
a there is a how sorry I don't know what
this is doing right
now there is a how to microp pipet and
an introduction on how to set a
micropipe header and how to do the
microp pipe heading and some activities
the teacher can use for that in the
molecular rainbow lab the students will
actually take various dieses that the
teacher has given them and the gel will
be set up so that the wells of the gel
are halfway through the gel instead of
that one end or the other so that
they're also able to investigate the
polarity of the molecules that they're
putting into the gel uh when the gel
runs usually if it's running DNA as one
of the options DNA is a negative
molecule and gets pushed away from the
one side of the gel electropheresis
um unit and travels to The Far Side when
we put the wells in the center of the
gel the ones that are positive will
travel towards the negative end of the
electris machine and the ones that
are um negative will travel towards the
positive side of the electroforesis
machine so they'll go in opposite
directions based on their charges so
students will be able to predict charges
and see that charge is also one of the
ways that molecules can separate within
a gel and then they'll also be able to
talk about size of those dyes and be
able to see the component parts of those
dyes because they will separate just
like they did in the chromatography so
it's tying in what they've done
previously taking it to a higher level
and then adding in the component of
charge to it and then for
evaluation so in the evaluation part
they're going to do a gallery walk so
you can have students create Google
slides and share them where they can
present them to the class or you can do
just regular marker pencil and um chart
paper and have them both graph their
results from the
um Labs that they did both of electris
the chromatography and the exploration
using um the Google streets and have
them give their information so in in the
one they were trying to do their Test
full question and their hypothesis and
their their data and they can present
this as a post scientific poster
presentation uh for give feedback from
students they can give them sticky notes
and say hi you know I like your data
here um there's I I have questions about
this and they can rotate around the room
looking at each other's information if
they're doing a Google slide
presentation then they can still put
their sticky notes with the person's
name and then just stick it on this the
kid and so they can read all the
feedback and they can go back and and
re-edit their slides before submitting
it for uh final
grade and then these are some of our
references that the teachers can use as
well and there's handouts along with
that that both covers the lab and the
activities yep and then um that's it for
us okay
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