K3 (Kesehatan dan Keselamatan Kerja) Laboratorium Teknologi Pangan dan Hasil Pertanian

Kanal Pengetahuan Fakultas Teknologi Pertanian UGM

17 Aug 202106:58

Summary

TLDRThis video script emphasizes laboratory safety and health protocols for research smoothness. It advises reading lab rules, understanding chemical reagent properties and safety data, and using protective gear. It also covers the importance of cleaning work areas, knowing safety equipment locations, handling volatile chemicals in fume hoods, and proper waste disposal. Additionally, it highlights the need for sterilization of equipment and media in autoclaves, careful handling of glassware, and regular equipment checks to prevent accidents, concluding with the reminder to prioritize safety.

Takeaways

📚 Read and follow all laboratory safety protocols before starting work.
🧪 Always review the Material Safety Data Sheet (MSDS) for each chemical reagent used.
👔 Properly wear laboratory coats and ensure they are securely fastened to protect your body and clothing.
🧤 Use additional personal protective equipment (PPE) as needed, such as gloves, safety glasses, and masks.
👟 Wear comfortable and recommended closed-toe footwear to protect from chemical spills.
🧼 Always clean your work area before and after conducting experiments.
🚨 Be aware of the location of safety equipment such as fire extinguishers and first aid kits.
🌫 Use fume hoods properly when handling volatile chemicals to prevent inhalation.
🔥 Keep flammable solvents away from open flames like Bunsen burners.
🗑 Properly dispose of research waste according to its classification, such as non-hazardous, hazardous, and glass waste.
🔬 Be cautious when using glassware to prevent accidents and dispose of broken glass in designated containers.
💡 Ensure that gas cylinders are safely secured with regulators, bodies, and pressure checks before and after use.
🔌 Perform visual inspections on electrical equipment before use and avoid using unsafe equipment.
📝 Always update the logbook with details of equipment used and their condition after use.

Q & A

What is the primary purpose of the video?
-The primary purpose of the video is to demonstrate the application of K3 (Health, Safety, and Environment) principles in a laboratory setting to ensure personal safety and support smooth research operations.
What is the first thing one should do before starting work in a laboratory?
-Before starting work in a laboratory, one should read and pay attention to every safety regulation present in that laboratory.
Why is it important to read the Material Safety Data Sheet (MSDS) before working with chemical reagents?
-Reading the MSDS is important to understand the hazardous chemical symbols, properties, and incompatible materials of the reagents to ensure safety while handling them.
What should one do before using laboratory equipment?
-One should read the equipment's operating instructions carefully before using any laboratory equipment.
What is the requirement for laboratory coats when working in a lab?
-Laboratory coats must be worn correctly at all times while working in the lab, with all fasteners securely closed to protect the body and clothing effectively.
What additional personal protective equipment might be required in a laboratory?
-Additional personal protective equipment may include gloves to protect from organic solvents, heat-resistant gloves, and masks for those using kerudung (headscarves). Hair should be tied back neatly if not covered by a kerudung.
Why is it necessary to clean the work area before and after working in a laboratory?
-Cleaning the work area before and after working is necessary to prevent accidents and contamination, ensuring a safe and sterile working environment.
What should be considered when disposing of research waste?
-Research waste should be separated according to its type, such as non-hazardous waste, hazardous waste, and glass waste, and disposed of properly.
What precautions should be taken when using flammable solvents near open flames?
-Flammable solvents should be kept away from open flames like a Bunsen burner to prevent fires or explosions.
What is the procedure for using an autoclave?
-Before using an autoclave, ensure there is enough water, close the autoclave tightly, set the temperature and time according to the needs, and turn on the machine. After autoclaving is complete, check that the pressure has returned to normal and the temperature has reached approximately 50 degrees Celsius before handling the sterilized items with heat-resistant gloves.
Why is it important to log the use of equipment in a logbook?
-Logging the use of equipment in a logbook is important for tracking the equipment's usage, maintenance, and condition, which aids in maintaining safety and functionality.
What is the final reminder given in the video regarding safety in the laboratory?
-The final reminder is to always prioritize workplace safety when working in a laboratory.

Outlines

00:00

🧪 Lab Safety and Health Measures

This paragraph emphasizes the importance of adhering to safety protocols in the laboratory to ensure personal safety and support smooth research processes. It outlines the necessity of reading and following laboratory rules, understanding the properties of chemical reagents, and wearing appropriate personal protective equipment (PPE) such as lab coats, gloves, and masks. Additionally, it stresses the importance of cleaning the workspace before and after work, knowing the location of safety equipment, and handling volatile chemicals with care. The paragraph also covers the proper use of equipment like autoclaves and the segregation of research waste.

05:00

🔬 Safe Laboratory Practices

The second paragraph focuses on specific safety measures to be observed in the lab. It advises against using damaged glassware and disposing of broken pieces properly. It also highlights the importance of checking the safety of gas cylinders and electrical equipment before use. The paragraph stresses the need to keep the workspace tidy and safe to prevent accidents and to always update the equipment log book with personal data and the condition of the equipment used. It concludes with a reminder to prioritize workplace safety in the laboratory.

Mindmap

Keywords

💡K3

K3 refers to 'Keselamatan, Kesehatan, dan Kegiatan' in Indonesian, which translates to 'Safety, Health, and Activity' in English. It is a concept that emphasizes the importance of safety and health in the workplace. In the context of the video, K3 is crucial for laboratory safety protocols to ensure that researchers and staff are protected from potential hazards while conducting experiments. The script mentions applying K3 in the laboratory to maintain safety and support smooth research.

💡Laboratory Safety

Laboratory Safety is a set of practices and protocols designed to protect laboratory workers from accidents, injuries, and health hazards. The video script underscores the importance of adhering to laboratory rules and guidelines to ensure personal safety and facilitate research. This includes understanding chemical properties, using personal protective equipment, and maintaining a clean workspace.

💡Material Safety Data Sheet (MSDS)

An MSDS is a document that contains information on the properties of a chemical substance, including potential hazards, how to handle and store it safely, and emergency procedures. In the script, it is mentioned that before working with chemical reagents, one should read the MSDS to understand the hazardous symbols and properties of the chemicals, which is a critical step in laboratory safety.

💡Personal Protective Equipment (PPE)

PPE refers to specialized clothing and equipment worn by workers to protect themselves from potential hazards. The video script mentions wearing laboratory coats properly, using gloves, and other protective gear like masks and shoe covers. These are essential to protect the body from chemical splashes and other laboratory hazards.

💡Chemical Compatibility

Chemical Compatibility refers to the ability of different chemicals to be mixed or stored together without causing adverse reactions. The script advises paying attention to the properties of chemical substances, including incompatible chemicals, to prevent dangerous reactions in the laboratory.

💡Autoclave

An autoclave is a device used to sterilize equipment and materials by subjecting them to high-pressure steam. The script mentions the proper procedure for autoclaving, including ensuring there is enough water, wrapping items properly, and allowing the pressure and temperature to return to normal after the process. This is important for maintaining a sterile environment in the laboratory.

💡Laminar Flow

A laminar flow hood is a safety device that provides a sterile working environment by blowing filtered air horizontally across a workspace. The script mentions working in a laminar flow hood and ensuring that all equipment and body parts entering the hood are sterilized with disinfectant. This helps to prevent contamination of samples and maintains a clean laboratory environment.

💡Waste Disposal

Waste Disposal in a laboratory context involves the proper handling and disposal of hazardous waste materials. The script discusses separating research waste according to its type, such as non-hazardous waste, hazardous waste, and glass waste. This is crucial for environmental safety and compliance with waste management regulations.

💡Glassware

Glassware refers to laboratory equipment made of glass, such as beakers, flasks, and test tubes. The script advises using glassware with care to prevent accidents and to discard broken pieces in designated containers. Proper handling and disposal of glassware are essential for laboratory safety.

💡Electrical Safety

Electrical Safety involves the practices to prevent electric shock and fire hazards. The script mentions inspecting electrical equipment visually before use and not using unsafe equipment. This is important to prevent accidents and ensure the safety of everyone in the laboratory.

💡Log Book

A log book is a record-keeping system used to document the use and maintenance of laboratory equipment. The script emphasizes the importance of always filling out the log book after using equipment, recording personal data, and the condition of the equipment. This practice helps in tracking the usage and maintenance history of equipment, ensuring its proper functioning and safety.

Highlights

Welcome to the safety and health at work video by the Department of Occupational Safety and Health.

Demonstrates the implementation of K3 in the laboratory to ensure personal safety and support smooth research.

Each laboratory has its own rules that must be followed.

Read and pay attention to each rule before starting work in the laboratory.

Ensure to read the Material Safety Data Sheet (MSDS) before working with chemical reagents.

Pay attention to the hazardous chemical symbols on the reagents.

Consider the properties of the chemical substances, including incompatible chemicals.

Read the equipment instructions carefully before using laboratory tools.

Laboratory coats must be worn properly during work.

Protect your body and clothes by wearing additional personal protective equipment as needed.

Tie back hair neatly and wear comfortable, closed shoes to protect from chemical spills.

Always clean the work area before and after working.

Be aware of the location of safety equipment in the laboratory.

When using volatile reagents, perform evaporation in a fume hood.

Ensure all equipment and body parts entering the laminar flow hood are sterilized with disinfectant.

Keep flammable solvents away from open flames like Bunsen burners.

Dispose of research waste according to its category: non-hazardous, hazardous, and glass waste.

Before starting the autoclave, ensure there is enough water and close the autoclave tightly.

Set the temperature and time according to the needs and turn on the autoclave.

After autoclaving is complete, ensure the pressure has returned to normal and the temperature has reached approximately 50 degrees Celsius.

Use heat-resistant gloves to handle equipment and media that have been autoclaved.

Do not dispose of microbial culture waste directly but autoclave it first at 121 degrees Celsius for 20 minutes.

In the laboratory, pay attention to the use of glassware to prevent work accidents.

Ensure the safety of gas cylinders such as regulators, bodies, and pressure before and after use.

For electrical equipment, perform a visual inspection before use and do not use equipment that is unsafe.

Ensure that tools and materials in the work area are tidy and safe to prevent work accidents.

After using tools, always fill in the logbook with personal data and the last condition of the tools used.

Always prioritize safety when working in the laboratory.