Perbanyak Tanaman Dengan Kultur Jaringan

Kementerian Pertanian RI
25 Nov 202012:30

Summary

TLDRThe video covers a training session on plant tissue culture techniques aimed at enhancing the quality and variety of ornamental plants. The session highlights the use of hormones, mutation agents, and inhibitors in plant propagation, emphasizing biotechnology's role in boosting income for communities. The video also discusses the challenges of tissue culture, including sterilization and contamination issues. Additionally, it features a practical demonstration on home-scale tissue culture and promotes the accessibility of these techniques to all, including women and students, for widespread societal benefit.

Takeaways

  • 🌱 Tissue culture is a method for plant propagation that can produce high-quality and varied plants, especially ornamental ones.
  • 🌍 Certain plants like the Panda have gone extinct in the wild, and tissue culture is a means to conserve such species.
  • 🧪 The use of hormones, mutation agents, and growth inhibitors in tissue culture can produce diverse and high-quality plant varieties.
  • 🏡 The technique of tissue culture can be practiced at a household scale using basic tools like enclosures and open spaces.
  • 👩‍🏫 Training and practical application are essential for successful tissue culture, even for those without advanced education.
  • 💡 The 'Inti-Plasma' program is a concept designed to simplify the complex aspects of tissue culture for broader community use.
  • 🤝 Collaboration with local farmers and government support has enabled the spread and application of tissue culture techniques in communities.
  • 🚜 Specific crops like Philodendron Whitelight are being cultivated for export using tissue culture, with flexible production based on market trends.
  • 🔬 The key challenges in tissue culture include the sterility of the environment and the risk of contamination during the process.
  • 🎓 The Esa Flora initiative provides opportunities for students and the broader community to learn and practice tissue culture, aiming to enhance the agricultural sector's productivity.

Q & A

  • What is the primary focus of the training mentioned in the script?

    -The primary focus of the training is on plant propagation techniques, specifically using tissue culture, hormones, mutation-inducing agents for variegation, and growth inhibitors to produce high-quality ornamental plants.

  • Who is leading the training session described in the script?

    -The training session is led by Edi Sandra, a lecturer who is currently working from home and applying biotechnological principles to community-based projects.

  • What challenges do people face when working with tissue culture, according to the script?

    -The script highlights that tissue culture is often perceived as difficult and expensive, particularly for lower-income communities. However, with proper training and guidance, even individuals with minimal education can learn the techniques successfully.

  • What is the concept of the 'inti-plasma' program mentioned in the script?

    -The 'inti-plasma' program is a concept where the complex aspects of tissue culture technology are handled by experts, while the simpler tasks are carried out by trained individuals, such as housewives, allowing them to contribute without needing to understand the full process.

  • How does the training benefit communities, according to Edi Sandra?

    -The training aims to help communities improve their livelihoods by integrating biotechnology into their practices, thus boosting their income through the production and commercialization of high-quality ornamental plants.

  • Which plant is highlighted as a popular export product in the script?

    -The Philodendron 'White Knight' is highlighted as a popular export product, currently in demand and propagated through tissue culture.

  • What is a significant issue encountered during tissue culture, as mentioned in the script?

    -A significant issue in tissue culture is contamination, which can occur if the explant, environment, or tools are not sterile during the process.

  • How does the script describe the role of students in the tissue culture project?

    -Students are given open access to participate in the tissue culture project, allowing them to practice and learn the techniques hands-on, contributing to the dissemination of this technology.

  • What are the steps involved in the tissue culture process as outlined in the script?

    -The tissue culture process involves selecting and preparing the explant, sterilizing it, initiating culture, incubating it under controlled conditions, and finally growing the plant in a sterile environment.

  • What future aspirations are mentioned for the tissue culture initiative in the script?

    -The script expresses hope that the tissue culture initiative will continue to expand and become widely adopted in agriculture, horticulture, and conservation efforts across Indonesia.

Outlines

00:00

🌱 Introduction to Tissue Culture and Its Applications

The speaker introduces the concept of tissue culture, specifically focusing on ornamental plants. He discusses the importance of modern techniques like hormone use, variegation, and inhibitors in enhancing plant quality and variety. The speaker highlights his background as an educator and his involvement in bringing biotechnology to the public. He emphasizes how tissue culture and biotechnology can boost the income of people, especially in the context of the rising popularity of ornamental plants like aglaonema, philodendron, and anthurium.

05:02

🌿 Practical Applications and Benefits of Plant Tissue Culture

The speaker explains the practical side of plant tissue culture, using the example of philodendron whitelight, a popular export plant. He outlines the tissue culture process, including initiation and scaling, and how it can be paused and resumed based on market demand. The speaker notes the challenges in tissue culture, particularly in handling contamination and ensuring sterile conditions. He discusses the importance of proper training and setting realistic expectations for those engaging in tissue culture, particularly for the less educated.

10:07

💡 Expanding Tissue Culture Knowledge and Its Impact on Society

The speaker details the process of teaching tissue culture to various groups, including farmers and students, through hands-on training and community programs. He shares success stories of how this training has empowered local communities, particularly women and youth, to engage in plant tissue culture. The speaker also discusses the long-term goals of making tissue culture more widespread and accessible, suggesting that it could play a significant role in agriculture, horticulture, and forestry. He expresses hope that government bodies will support the further development and commercialization of tissue culture technologies.

Mindmap

Keywords

💡Kultur Jaringan

Kultur jaringan refers to a technique in biotechnology where plant cells, tissues, or organs are grown in a controlled environment to produce new plants. In the video, this technique is used to propagate ornamental plants, demonstrating its potential for large-scale production and conservation. The speaker emphasizes the value of kultur jaringan in creating plant variations and high-quality specimens, which can be beneficial for both commercial and conservation purposes.

💡Eksplan

Eksplan is a piece of plant tissue used as the starting material for tissue culture. In the video, the speaker describes the importance of selecting the right part of the plant, such as the shoot apex or meristem, for successful tissue culture. The selection and sterilization of the eksplan are critical steps to avoid contamination and ensure healthy plant growth.

💡Sterilisasi

Sterilisasi is the process of making the plant tissue and the environment free of contaminants before placing it in a culture medium. In the context of the video, sterilisasi is a crucial step in tissue culture to prevent contamination, which is a major challenge in the process. Proper sterilization of the explant, tools, and environment is necessary to ensure the success of the culture.

💡Inisiasi

Inisiasi is the initial phase in tissue culture where the explant is introduced into the culture medium. The video explains that after the explant is sterilized, it undergoes inisiasi, which involves placing it into a sterile environment to start the tissue culture process. This step is crucial for the explant to begin developing into a new plant.

💡Bioteknologi

Bioteknologi refers to the use of biological processes for industrial and other purposes, especially the genetic manipulation of microorganisms for the production of antibiotics, hormones, etc. The speaker in the video highlights the role of biotechnology in enhancing plant propagation techniques like tissue culture, which can boost production quality and variety, thereby supporting both economic and conservation goals.

💡Varigata

Varigata refers to a mutation or variation in plants that results in variegated leaves, which are often highly valued in ornamental horticulture. In the video, the use of substances to induce variegation is mentioned as a method to create visually appealing and diverse plants. This technique is part of the broader effort to increase the commercial value and aesthetic appeal of ornamental plants.

💡Aklimatisasi

Aklimatisasi is the process of gradually adapting tissue-cultured plants to external conditions before they are planted in soil. The video discusses how plants like anthurium and aglonema, once grown in culture, must undergo acclimatization to transition from the controlled environment to natural conditions, ensuring they survive and thrive when planted.

💡Plasma Nutfah

Plasma Nutfah refers to the genetic material of plants that is conserved for future use in breeding and research. The video discusses the concept of a core-plasma program where challenging aspects of tissue culture are handled centrally, while simpler tasks are delegated to trained individuals. This approach helps preserve genetic diversity while making the technology accessible to local communities.

💡House Plant

House plants are plants that are grown indoors, primarily for decorative purposes. The video mentions the rising popularity of house plants such as aglonema, philodendron, and anthurium, and how tissue culture techniques are being used to meet the growing demand for these plants. The propagation of house plants through tissue culture is presented as a profitable opportunity in the horticultural market.

💡Kontaminasi

Kontaminasi refers to the presence of unwanted microorganisms that can spoil the tissue culture process. The video identifies contamination as a significant challenge in tissue culture, emphasizing the need for strict sterilization protocols. Contamination can lead to the failure of the culture, making it a critical issue to manage in the propagation of plants.

Highlights

Introduction to tissue culture techniques for ornamental plants using hormones and mutation inhibitors.

Tissue culture is presented as an innovative approach for conserving species that have become extinct in the wild.

Focus on practical, household-scale tissue culture techniques that can be done with simple equipment.

The speaker, Edi Sandra, shares his experience in applying and popularizing biotechnology among the community.

Emphasis on the commercialization of biotechnological advancements to positively impact society.

Discussion on the growing trend of ornamental plants, particularly philodendron, aglaonema, and anthurium.

The concept of 'inti-plasma' is introduced as a method to simplify complex biotechnological processes for community use.

Training programs are developed to empower even uneducated individuals to master tissue culture techniques.

The partnership with local farmers to enhance fruit production, with plans for future training and expansion.

Philodendron Whitelight is highlighted as a key export product, with tissue culture used for mass propagation.

Detailed steps of tissue culture are outlined, emphasizing the importance of sterilization to prevent contamination.

Esa Flora is an open platform for students and young people to learn and practice tissue culture.

The role of women in tissue culture is showcased, with examples of successful training outcomes.

Tissue culture is seen as a powerful tool for producing high-quality, large quantities of plants, with potential applications in various sectors.

Call for the integration of tissue culture into national agricultural and forestry strategies to enhance conservation and production.

Transcripts

play00:00

hai hai

play00:11

hai hai

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Hai ini adalah kultur jaringan kulit

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Panda atau review seventina dan ini

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sudah punah di alam liar karena sudah

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tahun 2009 di pasaran dan ini kita sudah

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mengkonservasi shareinfo dikutuk

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jaringan

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hai hai

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hai hai

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Hai assalamualaikum warahmatullah

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wabarakatuh Jadi Pagi ini kita akan

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melakukan pelatihan teknik perbanyakan

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tanaman hias dan up-to-date ya dengan

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menggunakan hormon dengan menggunakan

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zat mutasi varigata dengan menggunakan

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zat penghambat yang harapannya nanti

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akan menghasilkan tanaman-tanaman yang

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bervariasi dan berkualitas tinggi baik

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ya Kita siap-siap sekarang kita akan

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praktek Bagaimana teknik perbanyakan

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kultur jaringan dengan skala rumah

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tangga dan ini bisa dilakukan hanya

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dengan menggunakan enkas dan ditempat

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terbuka baik kita mulai hal

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[Musik]

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Hai nama saya Edi Sandra ya sehari-hari

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saya sebagai dosen Cuma sekarang sedang

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copied jadi lokfom jadi kita juga

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kebetulan di rumah kita berusaha untuk

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mengaplikasikan dan juga memasyarakatkan

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apa yang kita miliki yang kita dapatkan

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dari PBNU untuk ke masyarakat jadi kita

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memberikan contoh kepada masyarakat

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bahwa bioteknologi dan biodata Siti ini

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adalah sesuatu yang sangat baik untuk

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kita gabungkan Jadi bagaimana video

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posisi ini bisa kita tingkatkan

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kualitasnya manfaatnya dan

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komersialisasi Nya sehingga memberikan

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dampak yang sangat besar positif bagi

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masyarakat jadi saat ini sedang ramai

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orang membicarakan mengenai tanaman hias

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khususnya house plan jadi tanaman hias

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yang terkait dengan aglonema terkait

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dengan philodendron the

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Hai dengan anthurium dan sebagainya Oh

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itu sekarang sedang naik daun dan kita

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tidak hanya mengajarkan kepada

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masyarakat itu bagaimana berdagang tapi

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Kembali ke tadi tuh bagaimana asupan

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bioteknologi ini menjadi pendongkrak

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pendapatan mereka

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[Musik]

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Hai banyak orang mempertanyakan Iya

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kultur jaringan ini sangat sulit mahal

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dan sebagainya memang cocok buat

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masyarakat apalagi masyarakat bawah dan

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sebagainya nah Berdasarkan pengalaman

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kita yang tadi Sundari tahun 94 Memang

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betul sekali bahwa untuk masyarakat

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kecil itu tidak mungkin untuk bisa

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menelan itu sebuah menganalisa semua

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tapi kalau mereka diberi contoh dilatih

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mengerjakan mereka sanggup lagi bahkan

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lulusan sd pun atau tidak sekolah pun

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itu pegawai sehat yang itu-itu mereka

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terampil sekalipun di tidak jadi kalau

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mereka dilatih mengerjakan dia mereka

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bisa tapi jangan susah jangan disuruh

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untuk berpikir untuk menganalisa akan

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dipilih kalau hanya untuk melakukan bisa

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nah berkaitan dengan itu maka kita

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membuat satu konsep namanya program inti

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plasma

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Hai pengembangan putus jaringan

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berdasarkan inti-plasma semua teknologi

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itu kita setting yang susah-susah kita

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ngatur mereka hanya melaksanakan kayak

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ibu-ibu makodes itu gua mereka tampil

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memperbanyakkan di video tapi jangan

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disuruh memasarkan mereka hanya

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memperbanyak gitu udah hebat yang itu

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untuk membudidayakan novelnya hebat tapi

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ditaruh support Bagaimana memasarkan

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sebagian bra itu dibantu oleh link SH

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flora jadi asap warna pemasangan mereka

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sebagai ada pelatihan mereka sudah jadi

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instruktur Nah sekarang juga kita sedang

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mengembangkan pola-pola seperti itu kita

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sedang mendapatkan bantuan dari Dinas

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Pertanian Kabupaten untuk membantu

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kelompok tani tanaman buah khusus

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alpukat di suka hati itu mereka sudah

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ledakan rap dan sebagainya nanti program

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tahun depan Rencananya akan pelatihan

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tapi kita sudah jalan dengan petani

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mereka adiknya kita sudah pernah

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memberikan pelatihan mereka dan mereka

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semangat sekali

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Hai ini salah satu kultur jaringan

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tanaman ini yang lagi ngetop untuk

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Ekspor kita kerjasama yaitu tanaman

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philodendron whitelight gitu bagus untuk

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export nah ini setelah proses suka Pa

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inisiasi selama tiga bulan kemudian kita

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Perbanyaklah bentuk perbanyakan seperti

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ini nanti kita ada proses lagi

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pembesaran rusak Lim tapi kita juga bisa

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misalnya sekarang tiba-tiba lagi turun

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trennya kita gosok perbanyak ini

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disimpan dulu itu Nah itu mungkin

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mengetahui buatan lagi ngetop kita

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perbanyak lagi Jadi bisa didalam foto

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aman gitu ya selama setahun dua tahun ya

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contohnya seperti ini bisa sampai tiga

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tahun di sini kultur ada yang sampai 4

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tahun juga ada

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[Musik]

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I Ketut

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hai hai

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[Musik]

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please jadi tahapan kultur jaringan itu

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memang cukup panjangnya sehingga

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kembalikan itu banyak orang yang begitu

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mengetahui water nyata susah ya sebagai

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Bukan susah itu ya Tinggal bagaimana sih

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orang tadi itu kuat apa tidak bulat apa

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tidak melakoni setiap haven't read yang

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demikian teknis tadi itu Nah kalau bisa

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kita buat poin-poinnya jadi yang pertama

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sekali adalah dalam mencari bahan

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eksplan mencari bahan eksplan ini adalah

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mengharus tahu kita bagian tanaman mana

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yang bisa digunakan sebagai eksplan Nah

play07:19

itu Al terutama adalah bagian pucuk tapi

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calya kultur meristem dan juga satu

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bagian lateralnya Nah setelah kita ambil

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kemudian baru eksplan ini diproses

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diproses yang dan diprosesnya tadi itu

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ada dua sebenarnya untuk saya sinyal

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yang pertama ada sharecash diluar

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laminar

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Kemudian yang kedua adalah sterilisasi

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di dalam laminar itu proses sterilisasi

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itu lumayan itu proses setup yang

play07:44

panjang tadi itu nah selisih tadi itu

play07:47

gunanya apa karena bahwa si eksplan ini

play07:50

kan nanti akan memasuki botol yang

play07:52

steril jadi dia tadi itu saya eksplannya

play07:55

harus steril cara Namja harus steril

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orangnya harus steril lingkungan yang

play07:59

steril itu kenapa susahnya kultur

play08:01

jaringan kontaminasi itu menjadi

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permasalah utama dalam kultur jaringan

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tadi itu begitu kita tanam tadi itu

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setelah dispensasi kita tanam itu

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namanya inisiasi ya jadi demen cari

play08:14

eksplan Bruce kemudian sterilisasi tadi

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itu kemudian inisiasi Nah setelah

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inisiasi kemudian diinkubasi diinkubasi

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itu ditaruh di ruang-ruang rakutu tadi

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itu ditumbuhkan dalam suhu yang memadai

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tentunya dengan sinar yang memadai Nah

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itu dia bisa tumbuh dengan baik karena

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makanannya juga sudah kita setting tadi

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penuh

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hai hai

play08:36

Hi Ho

play08:37

hai hai

play08:41

Hai Nah untuk bisa memasyarakat Han ini

play08:48

makan kita Esa pora ini berusaha untuk

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menyebarluaskan seluas-luasnya jejaring

play08:56

kita itu sebagian besar adalah mahasiswa

play08:58

dan saya memang Memberikan suatu akses

play09:01

yang sebebas-bebasnya buat siswa dan

play09:05

mahasiswa se-indonesia boleh praktek di

play09:08

sini tidur ya Esa flora terbuka untuk

play09:11

generasi muda untuk belajar di SH flora

play09:19

Nuh saya jadi anggota KWT yang dipimpin

play09:30

oleh Pak Edhie itu dari 2011 di semua

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anggota KWT itu sama Pak Edi plat

play09:35

dilatih kultur jaringan kalau dan semua

play09:38

juga di BIP latihan dulu di

play09:41

Alhamdulillah Ibu di sini sebagian

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banyak udah bisa karena sama Pak Edi

play09:45

semua anggota KWT harus berlatih kultur

play09:48

jaringan Allah dengan bejaan waktu

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ibu-ibu di sini udah banyak yang bisa

play09:52

dargul jaringan itu ibu-ibu anak-anak

play09:56

daily dari anak SMP pun sampai mahasiswa

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bisa ini salah satunya Tanaman apa

play10:06

anthurium sirih Ya ini kemarin aklim

play10:10

dari botolan botolan dari tanaman yang

play10:15

saya tanam Alhamdulillah menjadi dari

play10:18

botolan

play10:18

udah jadi begini dan lagi ini Nah itu

play10:21

namanya aglonema rotundum dari botol

play10:25

se-kec Il ini tapi memang kalau kita

play10:29

rajin insya Allah bisa jadi itu

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Hai jadi saya berharap putih jaringan

play10:53

ini mampu memberikan daya dongkrak

play10:55

begitu ya buat Indonesia dan

play10:58

masyarakatnya karena kultur jaringan ini

play11:00

tadi itu memang merupakan gabungan dari

play11:04

teknologi-teknologi didalamnya yang

play11:06

mampu menghasilkan variasi tanaman juga

play11:11

apa bagaimana memproduksi dengan jumlah

play11:15

besar bagaimana menghasilkan yang

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berkualitas menghasilkan obat dan

play11:20

sebagainya nah ini semuanya Sangat hebat

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kalau diaplikasikan di masyarakat yang

play11:26

begitu hortikultura baik itu di

play11:29

perkebunan pertanian Kehutanan dan

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sebagainya jadi saya sih Berharap Bahwa

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Sakura ini terus bermuara

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4shared tekan dan inginnya sih bahwa

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kayak bisa Seperti dukun talenta itu

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pihak Kementerian kehutanan tuh bisa

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tadi cuma mengembangkan tadi itu yang

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konservasi invitro terus pihak pertanian

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bisa inilah mengadopsi bagaimana mengapa

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memasyarakatkan atau memproduksi bibit

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wujud hortikultura yang berkualitas

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unggul dan sebagainya dalam untuk

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misalnya industri tadi itu yang

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kecil-kecil kali itu sebagaimana kita

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menghitung mengembangkan kultur jaringan

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ini untuk buka-bukaan kecil tadi itunya

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itu kalau digabung itu hebat sekali

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[Musik]

play12:26

hai hai

play12:28

hai hai

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Plant TissueOrnamental PlantsBiotechnologyHorticultureCommunity TrainingSustainable PracticesPlant PropagationAgricultural InnovationHouseplantsGardening Techniques
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