UV Vis spectroscopy explained lecture
Summary
TLDRThis video tutorial from 'Somos Biology' delves into the fundamentals of UV-Visible spectroscopy, a technique widely used in chemistry and life sciences for determining the concentration of molecules in solutions. The script explains the setup of a spectrophotometer, the molecular mechanism behind light absorption, and how this technique can provide insights into chemical groups present in molecules. It also discusses the importance of 'lambda max' for identifying maximum absorbance and the use of the Beer-Lambert law for calculating unknown concentrations, making complex scientific concepts accessible to viewers.
Takeaways
- 🌟 UV-Visible spectroscopy is a widely used technique in both chemistry and life sciences for various experiments and analyses.
- 🔍 It is primarily used for determining the concentration of different substances or molecules in a solution, including cells in microbiology practicals.
- 📈 The technique can also provide some information about the chemical groups present in molecules, though the data is not highly reliable for differentiating between different types of groups.
- 🛠️ The setup of a UV-Visible spectrophotometer includes a light source, a monochromator to separate light wavelengths, a beam separator, and a detector to measure light intensity.
- 🌈 The UV-Visible range of the electromagnetic spectrum is from 200 to 800 nanometers, with UV ranging from 200 to 400 nm and visible light from 400 to 800 nm.
- 🔬 The molecular mechanism involves the absorption of light energy by molecules, causing electrons to jump from a ground state to a higher energy state, which is specific to certain wavelengths.
- 📊 Beer-Lambert Law is used to relate the absorbance of light to the concentration of a molecule in a solution, which is crucial for quantitative analysis.
- 📉 A plot of absorbance against concentration typically results in a non-linear curve, which can be linearized by using a logarithmic scale to facilitate easier calculation of unknown concentrations.
- 📚 Understanding the specific wavelength (lambda max) at which a molecule absorbs light most strongly is important for identifying the maximum absorbance for different chemical groups.
- 🔬 The molecular level process of UV-Visible spectroscopy involves the excitation of electrons from bonding or non-bonding orbitals to higher energy states, such as sigma or pi orbitals.
- 👍 The video concludes by emphasizing the importance of UV-Visible spectroscopy for concentration analysis and its limitations in identifying specific chemical bonds.
Q & A
What is UV-visible spectroscopy and why is it used?
-UV-visible spectroscopy is a technique widely used in chemical and life sciences to measure the concentration of different substances in a solution. It is also used to identify chemical groups present in molecules, although the data is not highly reliable for differentiating between different groups.
What are the main applications of UV-visible spectroscopy?
-UV-visible spectroscopy is used for determining the concentration of molecules in a solution, including in microbiology for measuring cell concentrations. It is also used in enzymatic reactions and enzyme kinetic studies to assess enzyme-associated activities.
How does the UV-visible spectrophotometry work?
-UV-visible spectrophotometry works by passing light through a sample and measuring the intensity of the light that is transmitted through it. The difference in intensity before and after the sample is used to calculate the absorbance, which is related to the concentration of the molecules in the sample.
What is the role of a monochromator in UV-visible spectroscopy?
-A monochromator is a device that separates the light from a source into its different wavelengths. It allows only a specific wavelength of light to pass through, which is then used to analyze the sample.
What is the significance of the wavelength in UV-visible spectroscopy?
-The wavelength is significant because different molecules absorb different wavelengths of light. By knowing the specific wavelength that a molecule absorbs, one can identify the presence of certain chemical groups within the molecule.
What is the relationship between frequency, wavelength, and energy of an electromagnetic wave?
-The frequency and energy of an electromagnetic wave are directly proportional; higher frequency means higher energy. The wavelength and frequency are inversely proportional; shorter wavelength means higher frequency and vice versa.
How is the concentration of a molecule in a solution determined using UV-visible spectroscopy?
-The concentration is determined by measuring the absorbance of the sample at a specific wavelength. Using the Beer-Lambert Law, which states that absorbance is proportional to the concentration of the molecule, the concentration can be calculated.
What is meant by 'transmittance' in the context of UV-visible spectroscopy?
-Transmittance is the ratio of the intensity of light after passing through the sample (I) to the initial intensity of light (I0). It indicates the amount of light that has been transmitted through the sample.
What is the difference between transmittance and absorbance?
-Transmittance measures the amount of light that passes through the sample, while absorbance measures the amount of light that is absorbed by the sample. They are inversely related; higher transmittance means lower absorbance and vice versa.
Why is it difficult to extrapolate data from a graph of percent transmittance versus concentration?
-It is difficult because the relationship between percent transmittance and concentration is not linear, making it challenging to predict the concentration of unknown samples from such a graph.
How can a graph of absorbance versus concentration be made linear for easier data interpretation?
-By plotting the logarithm of the concentration (or the reciprocal of transmittance) against absorbance, the graph becomes linear, which simplifies the process of extrapolating the concentration of unknown samples.
What is the molecular mechanism behind the absorption of light in UV-visible spectroscopy?
-The molecular mechanism involves the excitation of electrons within molecules from a lower energy state (ground state) to a higher energy state when they absorb energy from light of a specific wavelength. The energy absorbed corresponds to the difference in energy levels between the molecular orbitals.
Why are UV-visible spectra not reliable for identifying specific chemical bonds?
-UV-visible spectra are not reliable for identifying specific chemical bonds because many molecules with similar bonds and structures absorb light at similar wavelengths, making it difficult to distinguish between them based on UV-visible spectroscopy alone.
Outlines
🔬 Introduction to UV-Visible Spectroscopy
The video begins with an introduction to UV-Visible spectroscopy, a widely used technique in chemistry and life sciences for determining the concentration of molecules in a solution. The speaker explains that while this technique can provide some information about the chemical groups present in a molecule, it is primarily used for concentration analysis due to the reliability of its data. The video promises to delve into the setup, mechanism, and application of UV-Visible spectroscopy, including its use in colorimetry and enzymatic reactions.
🌟 Understanding the Setup of UV-Visible Spectrophotometry
This paragraph explains the basic setup of a UV-Visible spectrophotometer, starting with a light source that emits UV or visible light. A monochromator is used to separate the light into its individual wavelengths, creating a monochromatic beam. The beam separator directs this light towards two chambers, one for a control sample and one for the actual sample. The differences in light absorption between the two samples are then detected and recorded. The explanation includes the importance of wavelength and frequency in the context of the electromagnetic spectrum and how they relate to the energy of the light used in the technique.
📈 The Molecular Mechanism of UV-Visible Spectroscopy
The speaker describes the molecular mechanism behind UV-Visible spectroscopy, focusing on how molecules absorb specific wavelengths of light, causing electrons to jump from a ground state to a higher energy state. This absorption of energy results in a decrease in light intensity, which is measured by a detector. The concept of transmittance and absorbance is introduced, with the former being the ratio of light intensity after passing through a sample to the initial light intensity, and the latter being a measure of how much light is absorbed by the sample. The paragraph also touches on the importance of lambda max, the wavelength at which a molecule absorbs light most strongly.
📊 Data Interpretation in UV-Visible Spectroscopy
This section discusses how to interpret the data obtained from UV-Visible spectroscopy. The speaker explains the relationship between transmittance, absorbance, and concentration, and how a graph plotting absorbance against concentration can be used to determine the concentration of an unknown sample. The paragraph emphasizes the importance of using absorbance rather than transmittance for creating a linear graph, which facilitates the calculation of unknown concentrations. The explanation includes the Beer-Lambert Law, which quantifies the relationship between absorbance, the molar absorption coefficient, concentration, and path length.
🧬 Molecular-Level Understanding and Practical Application
The final paragraph delves into the molecular-level understanding of why molecules absorb light at specific wavelengths, discussing the energy states of different chemical bonds and how the energy from light is absorbed to excite electrons to higher energy states. The speaker also explains the limitations of UV-Visible spectroscopy in identifying specific chemical groups due to the similarities in the absorbance patterns of different molecules. The paragraph concludes with a reminder of the technique's primary use for concentration analysis and a call to action for viewers to like, share, and subscribe for more educational content.
Mindmap
Keywords
💡UV Visible Spectroscopy
💡Concentration Analysis
💡Molecular Mechanism
💡Monochromator
💡Beam Separator
💡Detector
💡Transmittance
💡Absorbance
💡Lambda Max (λmax)
💡Molar Absorption Coefficient (Epsilon)
💡Beer-Lambert Law
Highlights
UV visible spectroscopy is widely used in chemical and life sciences for various experiments.
The technique helps in determining the concentration of different molecules in a solution.
UV visible spectroscopy can also measure the concentration of cells in microbiology practicals.
The technique provides limited information on the chemical groups present in molecules.
The video explains the setup of UV visible spectrometry, starting with a light source.
A monochromator is used to separate different wavelengths of light into a single beam.
The role of a beam separator in directing light to control and sample chambers is discussed.
The detector measures the intensity of transmitted light, which is key to determining concentration.
The molecular mechanism involves electrons absorbing energy and jumping to higher energy states.
Sigma and pi bonds require different amounts of energy for electron excitation.
The video explains the concept of transmittance and absorbance in the context of spectroscopy.
Lambda Max is identified as the maximum wavelength for which absorbance is the highest.
The Beer-Lambert Law is used to relate the concentration of a molecule to its absorbance.
A graph of absorbance versus concentration can help determine the concentration of unknown samples.
The video demonstrates the process of creating a calibration curve for concentration analysis.
UV visible spectroscopy is not used for identifying specific chemical bonds due to limitations.
The video concludes by summarizing the practical applications and limitations of UV visible spectroscopy.
Transcripts
[Music]
welcome back friends welcome to another
video tutorial from somos biology and in
this video tutorial we want to talk
about UV visible spectroscopy I've got a
request from many of my subscribers to
make a video on that previously made
video on IR spectroscopy and extra NMR
spectroscopy so if you want to know
about NMR and IR spectroscopy you can
look at my channel and you will get
videos on that so let's begin with UV
visible spectroscopy UV visible
spectroscopy is very widely used
technique in not only chemical or
chemistry labs but also in life sciences
many different experiments to rely on
the idea of UV visible spectroscopy but
the first question is why we need this
technique if it's a technique then what
this technique is giving us the answer
to that is in many situation we need to
find concentrations of different
substances concentration of different
molecules in a solution and that we can
measure perfectly with UV visible
spectroscopy and in some cases also
concentration of cells during the
microbiology practicals we can also use
UV visible spectroscopy to measure so it
can give us a good data about the
concentration of any molecule that is
present in a solution but it also can
tell us a little bit about the chemical
group that is present in the molecule
but the data that we get is not very
much reliable because for the different
types of group the UV visible
spectroscopy can give us very similar
data so you generally use it for only
concentration analysis but how exactly
we will get to know the concentration
from the UV visible spectrophotometry
let's look at that in this video to
understand using visible spectrometry we
also tagged it with known as the
colorimetry which is linked something
with the different colors of the color
substances which can help us to identify
the concentration of the colored
compound that is present in there and we
use it extensively in enzymatic
reactions to find out enzyme
associated activities an enzyme kinetic
studies so the first thing that will
tell you is what easy visible
spectroscopy and how the whole technique
is oriented like how the mechanic the
mechanism of the technique so all these
instruments that are present there to
make this technique possible then I'll
tell you the molecular mechanism with
which this whole technique works so
let's begin with the actual setup of
this UV visible spectrometry and that
gives us start with a source that you've
definitely a light source because as we
see UV visible spectrometry utilizes UV
or visible range of light as the energy
source primary energy source to to find
out the important feature of
concentration in case of is spectrometry
we know that infrared is the range of
the light that they've used but in this
case we use UV or visible range and you
know UV and visible range like
electromagnetic range you should have a
little idea about the electromagnetic
wavelength that we see completely and
some basic idea before talking into the
details is if you look at the
electromagnetic spectrum you will find
out different wavelengths it's ranging
from like gamma rays to ultimately
ending there in the microwave as well as
infrared waves till the end so what
those different waves are telling us the
important feature you always need to
know is the idea of two things one is
the wavelength and the frequency of that
electromagnetic wave the more the
frequency of a wave the higher energetic
that wave is that energy is that's idea
more frequency more energy and if you
have more frequency the wavelength will
be long I mean so the less sorry more
frequency less wavelength higher energy
and less energy means less frequency and
longer wavelength so very long
wavelength that will give you less
frequency and less energy higher like
shorter wavelength more frequency more
energy so in this case if we divide this
UV visible spectrum into the small part
and fractions what we'll find here is
simply ranging me from
200 to 800 that's kind of the range
although we have we can extend it till
10 20 nanometer in the wavelength but
from 200 nanometer to 800 nanometer that
will be the range from 200 to 400
approximately taken as a UV range and
from 400 to 800 approximately taken here
for the visible light range that we
usually use for the UV visible
spectrometry but normally actually UV
range ranges even back less wavelength
less number of nanometer of the
wavelength till this part as well so
what we know here by looking at the
spectrum is that as we are going close
to UV the wavelength is shorter so the
frequency is higher as we going this
side the frequency is low so this right
hand side we find more like a like a
microwave IR or infrared that means long
wavelengths less energy less frequency
till we get towards UB x-ray gamma ray
that means very short wavelength and
frequency is very very high energy also
very very high this is the scheme now
from this scheme you only take the 200
nanometer to 800 nanometer range now in
this case whenever we go to 400 to 800
nanometer we have the visible range of
the light that is visible colors that we
know the big G or the color violet
ultraviolet that from the from the
violet till the red that is a different
7 separate colors are ranging from this
part of the visible range now what we do
now is simply if you look at the no
instruments it goes like the source okay
so let any kind of light source a
filament in there this is the light
source and after the light source it has
a separate unit known as a monochromator
let me draw this unit this is mono
chromatic what motor chromatid does is
normally you know the source light as it
will have visible and you arrange it
have this this basic light that we know
the law
coming from the lightbulb and a
headlight of your scooter so dancing 27
separate all these different wavelengths
so monochromator separates all those
different wavelengths of the light into
single beam that's why called mono
chromatic because if we make single beam
from all this mixture of this light and
after the monochromator what else we
have we have the beam separator and the
beam separator almost like like almost
what we can say simple norm which can
separate the beam and guide it into two
separate directions and in this two
separate direction we have two separate
chambers one is for the control and the
sample chamber and then right after both
this chamber both the chambers are
connected to a detector this is the
simplest drawing of any individual
spectrophotometer and it begins with the
with the source and the source light
comes in and here we have small small
gap slits through which the light passes
after passing there there will be this
this mirrors will reflect it but
ultimately there is a prism inside this
monochromator so this is prism so I have
this prism this prism will separate
those that light into all these
different types all these different
wavelength of the light different color
so only one specific wavelength will be
allowed to pass through this ultimate
chain now you can alter this like
anytime you can pass a red light you can
pass blue it can pass orange except a
scalar depends on in which angle are the
the channel is present through which if
we go to this beam separator and the
beam separator will separate the same
monochromatic light send it to two
chambers the Chamber's with the control
and the Chamber's with the sample and
then from both this they will get the
data
detector detection by the detector and
then finally they will record the data
and give you the answer to it now the
question is in the simplest structure
that we see why this kind of structure
is paper and what exactly our role in
this case the idea of UV visible
spectrometry is simply whenever the
light hits a specific wavelength of
light hits a molecule that molecule get
excited and the electron once excited is
jumped up from a lower or we can say
ground state to a higher energy state
and whenever the electron jumps out what
it does is absorb that particular light
energy because the electron utilizes
that energy to move from the ground
state to the top state right because you
know one thing all your light is that
energy cannot be created it cannot be
destroyed the only thing possible is
transforming energy from one form to the
other so in this case getting the energy
so energy is little bit absorbed at that
point energy is utilized at this point
is in a more scientific way so what we
know if the energy is utilized then what
we know the intensity of that light that
received is lost a little bit right so
what we know if we put a blank a little
cubic qubit are small chambers where we
add samples to date so generally they
are made a bit glass so this Gloves
cubed is present here and in this qubit
we only put water so there is no
molecules present there or nothing is
very completely basic water so what
happens that light beam passing through
water and water there is no molecule to
finally absorb any light or utilizing
the energy from the light so what it
does simply it usually passing almost
the same intensity that ultimately
reaches here so what we get here there
are two separate intensity that a
detector always can measure in both the
sku which we put water then what will
happen
same like that become so normally the
light that generally come if there is no
absorption known as the intensity of the
light starts with i0 this is the start
point of the intensity of the light
then if you put the sample then we get
another intensity depending upon the
molecule that is present in that sample
but here same so we also call it I
because that's the intensity of the
sample but in this case we know if you
put water in both the chambers then we
will get the value of i0 will be equal
to I will be seen so the intensity of
start and intensity at the end are same
that means we're dealing with same
solution and literally this solution is
not taking any intensity that is first
transmitted through this beam separator
but now imagine in the sample tube in
the sample chamber we put a qubit and
here what we put a solution let the
protein let's say put protein solution
in there now proteins mainly there are
bulky rings in the proteins and all
those aromatic amino acids those amino
acids will receive then we energy at a
specific wavelength and if you also put
DNA they will also be absorb a specific
wavelength of light compared to the
other like and that is also due to the
bases that are present in the DNA so
usually for proteins that we know that
it will absorb the 260 nanometers of the
wavelength so once you see the 250
nanometer of the wavelength here and
that causes absorption of that
wavelength absorption of few of this
energy by the number of protein
molecules that are present there the
amount of protein the concentration of
protein that is present there the more
proteins present there the more
absorption and the less is this
intensity of the incident and intensity
of the of the reflected eye because you
know light is passing reflected and
finally reaching the detector so the
more absorption there is I value will be
decreased so in that case we know there
is a net change in intensity and what we
can find about the net change is this
this is the net change in the intensity
is it it so what we know here as as
known as a transmittance transmittance
known as the value for so we call it
transmittance or capital T
the value for I by i0 that is known as a
transmittance so the more light is
refracted the more light is removed
that means the more transmission
transmission take place and the less
light is removed which is the value of I
will be less the less transmittance is
there so if we know the value of
transmittance now what is about
absorbance transmittance and absorbance
are opposite to each other
different to each other so transmittance
is I by I 0 in that case absorbance will
become a lot of value of i0 by I that's
how you measure absorbance so more
transmittance less absorbance less
transmittance more absorbance and that's
kind of a true and it depends on the
concentration of the molecules that is
present in the sample now what kind of
molecule that is present there dictates
also what kind of group chemical group
is present also dictates at which
wavelength they will receive the energy
that is also very very important and for
every single type of chemical groups
there is a specific wavelength for which
the absorbance is maximum known as a
lambda Max or lambda maximum lambda max
is the maximum wavelength for which they
will give you most absorbance and the
readings they're known as lambda max we
can also get the value of lambda max for
different types of chemical groups now
the question is why we they absorb this
light and how this process works
molecular level we'll talk about that in
a moment but before going in there let
me first tell you how exactly we
interpret this data because you know
detector detects two things v-0 and I
the difference between the two will tell
you whether they will absorb some light
or they transmitted more light so for
that what we can find in this particular
case is simply we can plot a data with
the the absorbance and the difference in
the absorbance by that percent like the
difference of the absorbance along with
the concentration so if you know one if
you know if you know the absorbance we
can also find the concentration
there but how exactly because we know
that the transmittance if we look
normally in this data they are not going
to give us absorbance as a value because
the detector detects the transmittance
right because the absorbance is there in
the sample and detector only detects how
much is transmitted how much transfers
through this Q weight to the detector so
always the value that we get will be in
the transmittance form so simply what we
can draw from here we can draw a graph
and in that graph what we say the
percent transmittance and also
concentration of the solution of the
molecule so transmittance will be almost
the value of 10 to the power minus
concentration into the length of the
qubit that is the length of the qubit
dictates also how and how many molecules
of that molecule is present in that
qubit so according to beer and Lombard
they told us that P our transmittance
can be measured in the form of 10 to the
power minus concentration into the
length but transmittance is not going to
give us much data because if we put the
data in the terms of transmitted wave
let's say the concentration is very less
transmission should be maximum right
absorbance in that case will be very
less so transmittance will be highest
then let's elevate them a little bit
more and more and more so what kind of
graph we get we get a graph like this
slope like this this is not a straight
line this is not a linear drop now the
problem with this graph is although we
are getting the data directly from the
detector as observed percent
transmittance and the concentration it
will be very difficult for us to
extrapolate the data let's say we know
samples and we utilize some case samples
of the concentration which we know and
we plotted the plot with it then what we
do then we work an unknown and it
tasting that unknown and somehow the
unknowingly here here she becomes very
difficult for us to finally calculate
what exactly the concentration of that
unknown it becomes really difficult but
if the if the data is always straight
line
it becomes very easy to predict the
concentration of any unknown sample and
that's exactly what we do normally in
lab if you are checking up a protein
concentration we first prepare standard
concentration of proteins that the
concentration of which we already know
and then what we do then we plot a data
with it a straight line and then the
unknown sample whenever we get the
absorbance by putting in the data we can
simply extrapolate the data to the
concentration to find the actual
concentration of that protein but how
exactly we'd get a straight line in the
curve that is a big problem and the only
way is possible if we get the data in
absorbance scaled because knowing
absorbance scale the data should be
something like that we'll start from
local sensation low absorbance high
concentration high absorbance and linear
relation but the transmittance is not a
linear relation so in this case to make
it a linear relation of any curved line
remember that in your lifetime you will
always do a log
so the log value of concentration in two
lengths that can give you the value of
absorbance that will give you the value
of observers and the log value that we
utilize here is simply utilizing as a
constant that is known as molar
absorption coefficient or epsilon into
concentration into length we also can
write it as epsilon concentration is
written as C length is length of the
cubed known as L so the formula comes
down to epsilon C l equals absorbance
this is the net absorbance formula ecl
derived from lambert bier equation and
from this equation what you can also
tell if you know absorbance because in
the experiments we can find absorbance
so if you know absorbance you can find
concentration so for a concentration it
will be absorbance divided by epsilon
into
and this is how we can calculate a
concentration of an unknown molecule
unknown molecule that can be protein din
or any other molecule out there by this
fashion what we can create we can create
a straight line of the graph we can
trial of easily predict the
concentration let's say this is the
straight line of the graph and we know
the unknown protein is sommore somehow
represented and present someplace like
here so the concentration is simply drag
and we find it here and in that case
instead of transmittance EDB absorbance
so that's how you get the concentration
value from the graph as well okay so
this in a sense is the mechanism of how
we use UV visible spectrometry and to
get the data out of it but the second
part is also left why and how it's
working in the molecular level remember
for all this type of molecules I told
you it for all these different varieties
of molecules there is only a specific
wavelength for which that molecule will
get some energy and absorb the energy
and do some transmittance of the energy
now what is that mechanism to understand
that let me do some of this
so what we know at this particular point
is all those different different
molecules of chemical groups like
different bondings inside so for any
molecule if we look at here it's simply
we can divide in their energy states and
energy state with that like the ground
states like the nonbonding state or N
and then we have pi PI star Sigma star
Sigma this is almost like the simplest
idea of how the energy states are looks
like an atom in this particular case we
have the Sigma Phi n which is a
nonbonding and then five-spice turn and
Sigma star these are also nonbonding but
these are the bonding so the idea is we
have carbon carbon this is a single bond
present between carbon and carbon this
is a PI bond so what happens normally
here in this case of carbon
sorry carbonyl carbon this is Sigma
very sorry so have it's a sigma bond
strong very much strong Sigma bond and
Sigma bonds require more energy to
create and break so here Sigma bond is
there so so the electron that is present
let's say here in this orbital here and
the electron whenever the receives a
specific energy it can jump up to the
Sigma star so it will jump from one
orbital to the other this is a ground
state to the higher energy state but the
problem is in the higher energy state
the bond will not be very much stable so
again you need to remove from that so
this is how the electrons jump from a
ground state to a higher energy state
for a sigma bond directly from Sigma to
Sigma star now they can also jump to
this nonbonding orbital but if it takes
only less energy
similarly if it's C double bond o in
that case we know this is a PI bond so
for a pipe on the journey will begin
from this PI and it will go to the PI
star and from the PI to the in now it
depends on how much energy available at
what is the exact wavelength of light
that's sitting that's why they rely on a
specific wavelength for excitation for
UV visible spectrum matrix always the
excitation of that molecule in response
to the energy that they receive in
response to the wavelength of light
that's hitting now if the wavelength of
light is shorter that means very much
high frequency high energy for a sigma
bond it will directly jump to the Sigma
star but for a PI bond if it's work for
a very like a comparatively less energy
it cannot push the electron to the PI
from PI to PI star instead it will push
from PI to the nonbonding orbital and
that's how the whole thing's worked and
based on that the absorbance also vary
because the energy taken for the
electron to jump from the ground state
to the energy state is lost that's the
energy which is absorbed rest of the
energy is transmitted out that's how we
find the transmittance okay so in a turn
this is the idea from looking at this
idea you can tell
we kind of can bein able to get an idea
about what kind of bonds that are
present and what kind of chemical groups
that are present in a molecule but in
reality there are so many molecules with
similar types of bonds and the same
length same formula of those molecules
you will be you cannot recognize we
cannot recognize the exact group by
looking at this technique that's why we
don't use it to get an idea about the
specific chemical bonds that are present
which we use for NMR which we'll use for
the a magnetic resonance spectroscopy as
well as in case of is spectroscopy but
not in case of UV visible you only keep
you be visible or the process of
concentration analysis of molecules okay
so that's it if you liked this video
please hit the like button and share
this video with your friends and
subscribe to my channel to get more and
more videos like that thank you
تصفح المزيد من مقاطع الفيديو ذات الصلة
🧪 Spectroscopie d'absorption UV-visible (avec @myMaxicours)
Comment utiliser la loi de Beer-Lambert ? Première Spécialité Physique Chimie Lycée
NMR spectroscopy
Introduction to infrared spectroscopy | Spectroscopy | Organic chemistry | Khan Academy
What is Gel Electrophoresis | Don't Memorise
X ray crystallography basics explained | x ray diffraction
5.0 / 5 (0 votes)