Indentifikasi Zat Pewarna Sintetik Pada Produk Minuman (POLTEKKES MUHAMMADIYAH MAKKASAR) #ON_TLM2020

Summary

In this video tutorial, Andi Devani guides viewers through the process of identifying synthetic dyes, specifically Rhodamine B, in soft drinks. The experiment involves using wool to extract dyes through a heating process and observing color changes when specific reagents are added. The procedure, conducted as part of a toxicology practical, provides a clear method to identify synthetic dyes like Rhodamine B, which is often suspected in red-colored drinks. After following the steps, the test results show that the tested drink samples are negative for Rhodamine B. This video is part of a medical laboratory technology competition.

Takeaways

• 😀 The video introduces Andi Devani, who discusses identifying synthetic dyes in beverages.
• 🥤 Synthetic dyes can be found in both powdered and packaged drinks, with some being harmful, like Rhodamine B.
• 🔬 The tutorial focuses on detecting synthetic dyes, particularly Rhodamine B, in beverages using a practical approach.
• 🧪 Rhodamine B is a harmful synthetic dye, and its natural color is red, making red-colored beverages suspect for containing it.
• 🧵 The detection method involves soaking wool threads in the beverage, heating them, and observing any color changes.
• 🥼 The equipment used includes measuring glasses, tweezers, wool threads, porcelain bowls, HCl solutions, and the beverage samples.
• 🎨 Wool threads are heated for 15 minutes to absorb the dye and then tested with different chemical reagents to see the reactions.
• 🔍 The tutorial emphasizes the use of HCl and NH4OH solutions to create a reaction that reveals the presence of Rhodamine B.
• 🧪 After several steps, including washing and drying the wool, the final results are compared to a positive control.
• 🏆 The video is part of the National Olympiad of Medical Laboratory Technology competition organized by a Polytechnic institution.

Q & A

What types of beverages does the speaker mention Galang enjoys?

Galang enjoys both brewed and packaged soft drinks.

What synthetic dye is specifically highlighted as harmful in the script?

Rhodamine B is highlighted as a harmful synthetic dye.

What is the purpose of the video presented by Andi Devani?

The purpose of the video is to demonstrate how to identify synthetic dyes in soft drink products.

What is the primary method used to detect synthetic dyes in the practical experiment?

The method involves using wool yarn to absorb synthetic dye from the beverage sample through heating, followed by observing color changes after adding reagents.

Which materials are required for the practical experiment?

Materials required include beakers, measuring glasses, tweezers, wool yarn, porcelain dishes, HCl 0.05 N, soft drink samples, NH4OH 12%, NaOH 10%, concentrated HCl, and concentrated H2SO4.

Why is wool yarn used in the experiment?

Wool yarn is used because it absorbs synthetic dyes during heating, allowing the detection of color changes when reagents are applied.

How is the beverage sample prepared before testing?

The beverage sample is acidified with 0.05 N HCl before testing. If the sample is solid or in powder form, it is dissolved in distilled water (aquades).

What reagents are applied to the wool yarn after it is washed?

After washing the wool yarn, reagents such as NH4OH 12%, NaOH 10%, concentrated HCl, and concentrated H2SO4 are added, each in five drops.

What is the significance of comparing the test result with the positive control?

Comparing with the positive control helps determine whether the beverage contains synthetic dyes like Rhodamine B based on the observed color change.

What conclusion was reached regarding the presence of Rhodamine B in the tested samples?

The conclusion was that the sample tested negative for containing Rhodamine B.